Assembly of lamins in vitro  

作　　者：MIN GUNG WEI XIANG JUN TONG BIN CHEN BOZHANG ZHONG FAN LIU MING XIAO DING ZHONGHE ZHAI(College of Life Sciences, Peking UniversityCollege of Chemistry and Molecular Engineering, PekingUniversity Beijing 100871, China.) 

出　　处：《Cell Research》1996年第1期11-22,共12页细胞研究（英文版）

摘　　要：After lamins A, B and C were isolated and purified from rat liver, their assembly properties were examined by electron microscopy and scanning tunneling microscopy using negative staining and the glycerol coating method,respectively By varying the assembly time or the ionic conditions under which polymerization takes place, we have observed different stages of lamin assembly, which may provide clues on the structure of the 10 nm lamin filaments. At the first level of structural organization, two lamin polypeptides associate laterally into dimers with the two domains being parallel and in register. At the second level of structural organization, two dimers associate in a half-staggered and atiparallel fashion to form a tetramer 75 nm in length. At the third level of structural organization, 4-10 lamin tetramers associate laterally in register to form 75 nm long 10nm filaments, which in turn combine head to head into long, fully assembled lamin filaments.The assembled lamin filaments are nonpolar.

关 键 词：Nuclear lamina LAMINS scanning tunneling microscopy assembly 

分 类 号：Q248[生物学—细胞生物学] Q243