HPLC梯度洗脱法同时测定金嗓散结胶囊中6种主要成分的含量  

Simultaneous determination of six main constituents in Jinsangsanjie capsules by HPLC gradient elution

作　　者：谭雄斯[1] 庞武耀[1] 

机构地区：[1]肇庆医学高等专科学校,广东肇庆526020

出　　处：《中国药物应用与监测》2017年第5期263-266,共4页Chinese Journal of Drug Application and Monitoring

基　　金：2016年肇庆市科技创新指导类项目(201624030406)

摘　　要：目的:建立同时测定金嗓散结胶囊中6种主要成分的HPLC梯度洗脱方法。方法:选用Venusil MP C_(18)色谱柱;流动相:乙腈(A)和0.03%磷酸溶液(B),梯度洗脱:0~9 min 15.0%A,9~16 min 15.0%→21.0%A,16~22 min21.0%→36.0%A,22~30 min 36.0%→55.0%A,30~37 min 55.0%→75.0%A,37~45 min 75.0%→15.0%A;流速:0.8mL·min^(-1);柱温:30℃;进样量:10μL;检测波长:210 nm(苦杏仁苷、哈巴苷)、280 nm(安格洛苷C、哈巴俄苷)和208 nm(24-乙酰泽泻醇A、23-乙酰泽泻醇B)。结果:苦杏仁苷、哈巴苷、安格洛苷C、哈巴俄苷、24-乙酰泽泻醇A和23-乙酰泽泻醇B分别在一定的线性范围内线性关系良好(r≥0.999 2);平均回收率96.77%~100.05%,RSD值0.60%~1.55%;精密度和重复性良好;室温下金嗓散结胶囊供试品溶液在12 h内稳定。结论:该方法操作简便,结果准确,可用于金嗓散结胶囊中6种主要成分含量的同时测定。Objective：To establish the quantitative method to determine six main constituents simultaneously in Jinsangsanjie capsules by HPLC gradient elution.Methods：The separation was performed on Venusil MP C18 chromatographic column by HPLC gradient elution.The mobile phase consisted of acetonitrile（A） and 0.03% phosphoric acid solution（B）.Gradient elution was as follows：0-9 min 15.0%A,9-16 min 15.0%→21.0%A,16-22 min 21.0%→36.0%A,22-30 min 36.0%→55.0%A,30-37 min 55.0%→75.0%A,37-45 min 75.0%→15.0%A.The flow rate was 0.8 mL·min^-1.The column temperature was set at 30 ℃.The sample quantity was 10 μL.The detection wavelength were set at 210 nm（amygdalin,harpagide）,280 nm（angoroside C,harpagoside） and 208 nm（24-acetate alisol A,23-acetate alisol B）.Results：Amygdalin,harpagide,angoroside C,harpagoside,24-acetate alisol A and 23-acetate alisol B had a good linear relationship in a certain linear range（r ≥ 0.999 2） respectively.The average recovery was 96.77%-100.05% and the corresponding RSD was 0.60%-1.55%.The precision and the repeatability were good.Test solution was stable at room temperature within 12 h.Conclusion：The method was reliable,simple.It could be used for the simultaneous determination of 6 main components in Jinsangsanjie capsules.

关 键 词：金嗓散结胶囊 苦杏仁苷 哈巴苷 安格洛苷C 哈巴俄苷 24-乙酰泽泻醇A 23-乙酰泽泻醇B 

分 类 号：O657.72[理学—分析化学] R286.0[理学—化学]