Construction of a Genome-Wide Mutant Library in Rice Using CRISPR/Cas9  

作　　者：Meng, Xiangbing Yu, Hong Zhang, Yi Zhuang, Fengfeng Song, Xiaoguang Gao, Songsong Gao, Caixia Li, Jiayang 

机构地区：[1]Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China [2]Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China [3]Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China [4]Beijing ViewSolid Biotechnol, Beijing 102206, Peoples R China [5]Univ Chinese Acad Sci, Beijing 100049, Peoples R China

出　　处：《Molecular Plant》2017年第9期1238-1241,共4页分子植物（英文版）

摘　　要：Dear Editor Rice （Oryza sativa L.） is one of the world＇s most important staple crops and a powerful model system for studying monocot spe- cies because of its relatively small genome, rich genomic re- sources, and a highly efficient transformation system. With the completion of rice genome sequencing, the challenge of the post-genomic era is to systematically analyze the functions of all rice genes. Gene knockout is a frequently used and effective strategy for achieving this goal. Thus, generation of large-scale mutants at the whole-genome level is of great value for both func- tional genomics and genetic improvement of rice. Traditionally,Dear Editor Rice （Oryza sativa L.） is one of the world＇s most important staple crops and a powerful model system for studying monocot spe- cies because of its relatively small genome, rich genomic re- sources, and a highly efficient transformation system. With the completion of rice genome sequencing, the challenge of the post-genomic era is to systematically analyze the functions of all rice genes. Gene knockout is a frequently used and effective strategy for achieving this goal. Thus, generation of large-scale mutants at the whole-genome level is of great value for both func- tional genomics and genetic improvement of rice. Traditionally,

分 类 号：Q78[生物学—分子生物学] S511.03[农业科学—作物学]