Pathogenic bacterium Vibrio harveyi: an endosymbiont in the marine parasitic ciliate protozoan Cryptocaryon irritans  

作　　者：QIAO Ying WANG Jun MAO Yong LIU Min CHEN Ruanni SU Yongquan KE Qiaozhen HAN Kunhuang ZHENG Weiqiang 

机构地区：[1]State Key Laboratory of Breeding of Larimichthys crocea [2]College of Ocean and Earth Sciences, Xiamen University

出　　处：《Acta Oceanologica Sinica》2017年第10期115-119,共5页海洋学报（英文版）

基　　金：The National Natural Science Foundation of China under contract Nos 31372504,41176115 and 41476118

摘　　要：Vibrio harveyi, known as a pathogenic bacterium caused severe secondary bacterial infections of the large yellow croaker Larimichthys crocea, was identified as an endosymbiont in the marine parasitic ciliate protozoan Cryptocaryon irritans. Meta 16 S sequencing method was used to identify the bacterial flora in C. irritans, and V.harveyi was isolated via culture-dependent method. Vibrio harveyi was observed in cytoplasm of C. irritans at the stage of tomont both by transmission electron microscopy and by Fluorescence in situ hybridization; no signal,however, was detected in nucleus area. The relationship between V. harveyi and C. irritans and the role of endosymbiotic V. harveyi in C. irritans merit further investigation.Vibrio harveyi, known as a pathogenic bacterium caused severe secondary bacterial infections of the large yellow croaker Larimichthys crocea, was identified as an endosymbiont in the marine parasitic ciliate protozoan Cryptocaryon irritans. Meta 16 S sequencing method was used to identify the bacterial flora in C. irritans, and V.harveyi was isolated via culture-dependent method. Vibrio harveyi was observed in cytoplasm of C. irritans at the stage of tomont both by transmission electron microscopy and by Fluorescence in situ hybridization; no signal,however, was detected in nucleus area. The relationship between V. harveyi and C. irritans and the role of endosymbiotic V. harveyi in C. irritans merit further investigation.

关 键 词：endosymbiotic bacterium protozoan Vibrio harveyi Cryptocaryon irritans Meta16S sequencing 

分 类 号：S941[农业科学—水产养殖]