改进离体豚鼠工作心脏标本及眼镜蛇心脏毒素的作用  

IMPROVEMENT ON ISOLATED GUINEA PIG WORKING HEART PREPARATION AND EFFECT OF COBRA CARDIOTOXIN

作　　者：黄守坚[1] 陈克敏[1] 吴楚坤[1] 孙家钧[1] 

机构地区：[1]中山医科大学药理教研室,广州510089

出　　处：《中国药理学通报》1991年第2期105-109,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目No 346

摘　　要：离体豚鼠工作心脏用改良Krebs-Henseliet(KH)液灌注,控制O_2和CO_2流量使KH液的Pco_2在4.7～7.3kPa,HR,CO,AF,CF,LVP,dP/dt_(max),LVPW及做功效率在2h内基本稳定。1～10mg/L眼镜蛇心脏毒素(CTX)对离体工作心脏的作用表现为AF,CO,LVPW,LVP,dP/dt_(max)和LVEDP显著增加,CF减少,MVO_2和做功效率增加不显著,HR保持不变。Usually experiments done on isolated working heart preparation must be completed within 1 h, because of declining of cardiac functions after going beyond that limit reported by many authors. Our experiments on guinea pig showed that the viability time lasted at least 2 h. All measured indices of cardiac performance, namely LVP, dP/dtmax, MASP, HR, CF, AF, CO, LVPW, MVO2 and. working efficiency, remained stable within that period of time, .when PcO2 and PO2 of perfusing solution were adjusted at 4.7~7.3 kPa and over 67 kPa respectively, resulting in stability of pH value in 7.4 or so. Modification of the Krebs solution by devoid of phosphate and addition of pyruvate and EDTA may also be responsible for prolongation of the viability time. After such improvement, the preparation will give sufficient time to study the effect of drugs. Its use will be greatly widened. 1 -10 mg/L cobra cardiotoxin increased CO, AF, LVEDP, MASP, LVP, dP/dtmax, decreased CF, and unchanged HR.MVO2 and working efficiency were insignificantly increased. These results obtained from this preparation were in accordance with that from other isolated cardiac preparations.

关 键 词：心脏毒素 心肌收缩 离体心脏 

分 类 号：R965.2[医药卫生—药理学]