免疫组织化学双标在肿瘤侵犯判断中的应用  

Application of immunohistochemical double staining in detection of tumor invasion

作　　者：林蓁 刘德纯 杨文圣 

机构地区：[1]厦门大学附属成功医院病理科,厦门361003

出　　处：《中国组织化学与细胞化学杂志》2017年第5期514-518,共5页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的探讨免疫组织化学双标法在判断肿瘤侵犯中的应用。方法应用以DAB和快红(fast red,FR)作为呈色剂的En Vision法免疫组织化学双标技术标记肿瘤组织33例,观察肿瘤组织中肿瘤细胞、血管淋巴管与神经及其相互关系。结果肿瘤组织中肿瘤细胞被标记成棕色(DAB显色),血管淋巴管内皮与神经纤维被标记成玫瑰红色(FR显色);可疑肿瘤侵犯血管淋巴管的30例中,有28例可见标记成棕色的肿瘤细胞侵入标记成瑰红色的血管、淋巴管中(肿瘤侵犯血管淋巴管阳性),阳性率为93.3%;可疑神经侵犯的12例中,有10例可见标记成棕色的肿瘤细胞侵入标记成瑰红色的神经纤维中(肿瘤侵犯神经纤维阳性),阳性率为83.3%。结论以DAB和快红作为呈色剂的免疫组织化学双标法观察肿瘤是否侵犯血管淋巴管与神经组织,结果清晰可靠,可作为判断肿瘤有无侵袭性生长的重要依据。Object To explore the application of double-stain immunohistochemical method in detection of tumor invasion. Method 33 cases of tumor tissue specimens were double stained using EnVision method in which DAB and Fast Red were the substrates and chromogens. The method was evaluated based on its ability to distinguish tumor cells from surrounding vascular or lymphatic vessels and nerve fibers. Result In tissue sections, tumor cells were stained brown （DAB）, while vascular endothelium and nerve fibers were stained rose red （FR）. Among 30 cases that likely had tumor invasion into vascular and lymphatic vessels, 28 cases were found to have brown tumor cells visible in rose red regions of vascular and lymphatic vessels, resulting a positive rate of 93.3%. Among 12 cases that were suspected to have nerve fiber invasion, 10 were found to have tumor cells staining brown presence in nerve fibers staining rose red, giving a positive rate of 83.3%. Conclusion Double-stain immunohistochemical method with DAB and Fast Red as chromogens could be used to evaluate tumor invasion of nerve tissues, vascular and lymphatic vessels as well as provide measurement for its invasive growth.

关 键 词：疫组织化学 双标法 肿瘤侵犯 

分 类 号：R322.74[医药卫生—人体解剖和组织胚胎学]