枯草芽孢杆菌subtilis str.168双精氨酸途径分泌蛋白预测及功能分析  

Prediction and Functional Analysis of Secreted Proteins of Twin-Arginine Translocation Pathway in Bacillus subtilis subsp. subtilis str.168

作　　者：朱德全[1] 韩诚武[2] 尹红[1] 张路路[1] 白雪 姚嘉[3] 邢雷 张跃华[1] 卢伟[3] 栾积毅[3] 刘向东[3] 

机构地区：[1]佳木斯大学理学院,黑龙江佳木斯154007 [2]佳木斯大学生命科学学院,黑龙江佳木斯154007 [3]佳木斯大学机械工程学院,黑龙江佳木斯154007

出　　处：《安徽农业科学》2017年第30期128-130,163,共4页Journal of Anhui Agricultural Sciences

基　　金：黑龙江省教育厅科研项目(2016-KYYWF-0544)

摘　　要：[目的]对枯草芽孢杆菌subtilis str.168双精氨酸转运途径(Tat)分泌蛋白进行预测和功能分析。[方法]从NCBI中选取枯草芽孢杆菌subtilis str.168基因组注释的蛋白质氨基酸序列,然后用Signal P 4.0、Lipo P、Tat P、TMHMM 2.0软件分析该基因组中双精氨酸途径的分泌蛋白,同时采用COG功能数据库对预测的分泌蛋白进行功能注释和聚类分析。[结果]通过分析发现108个有motif没有酶切位点的Tat信号肽蛋白、25个有酶切位点没有motif的Tat信号肽蛋白、124个既有酶切位点也有motif的Tat信号肽蛋白,其中105个蛋白归为Tat途径的分泌蛋白。[结论]对枯草芽孢杆菌Tat途径分泌蛋白的基因组预测和功能分析,将为分析该菌胞外蛋白的分泌机制打下基础。[ Objective]Prediction and functional analysis of secreted proteins of Twin-Arginine Translocation （Tat） pathway Bacillus subtilis str. 168 were conducted. [Method] The genome sequences of B. subtilis subsp. subtilis str. 168 were used to predict to secretory proteins of Tat pathway and functional analysis. SignalP 4.0, LipoP, TatP, TMHMM 2. 0 were used to predict secretory protein of Tat pathway. Function of secretory proteins was also analyzed by COG （Cluster of Orthologous Groups of proteins） database. [ Result] There were 108 Tat signal pep-tides proteins containing motif without cleavage site of enzyme, 25 proteins containing cleavage site of enzyme without motif, 124 proteins con-taining cleavage site of enzyme and motif. 105 proteins were classified as proteins secreted by Tat pathway in these proteins. [ Conclusion ] These results will lay the foundation for analyzing the secretion mechanism of extracellular protein in B. subtilis.

关 键 词：枯草芽孢杆菌 分泌蛋白 双精氨酸分泌途径 功能分析 

分 类 号：S188[农业科学—农业基础科学]