盐芥EsABI1基因克隆及其功能预测  

Cloning of EsABI1 of Eutrema salsugineum and Its Function Prediction

作　　者：谷彩红 赵宝添[1] 高世庆 陈家红 张荃[1] 

机构地区：[1]山东师范大学生命科学学院,山东济南250014 [2]北京杂交小麦工程技术研究中心,北京100097

出　　处：《安徽农业科学》2017年第30期134-139,211,共7页Journal of Anhui Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR2014CM041);北京市自然科学基金项目(6162009)

摘　　要：[目的]对盐芥EsABI1基因进行克隆及功能预测。[方法]克隆盐芥EsABI1基因c DNA序列,对其蛋白保守结构域和系统进化进行分析。[结果]EsABI1与At ABI1属于进化上的同一分支,有最近的亲缘关系,都含有包含PP2C蛋白磷酸酶催化活性位点在内的11个保守功能域。定量PCR检测发现,盐芥EsABI1沉默植株中EsABI1表达水平较野生型均有不同程度的降低。60μmol/L ABA分别处理EsABI1沉默植株0、3和6 h后,发现EsABI1基因表达水平受ABA诱导上调,而且相比于盐芥野生型,EsABI1沉默植株中EsABI1受ABA诱导上调表达更加明显。[结论]EsABI1是ABA信号转导途径中的负调控因子,参与了盐芥对环境胁迫的响应。[ Objective ] To clone the EsABI1 of Eutrema salsugineum and predict the function. [ Method ] The cDNA sequence of E sAB I 1 of E. sal-sugineum was cloned to study the protein conserved domain and phyletic evolution. [ Result] EsABIl and AtABIl had highest similarity and be-longed to one branch in evolution,which contained 11 conserved domains, including PP2C protein phosphatase catalytic active site. Quantitative PCR detection showed that the expression level of E sAB I1 in EsABI1 silent plants was lower than that in the wild type. The expression of EsABI1 had been up-regulated under 60 （jimol/L ABA for 0,3,6 hours in E. salsu gin eum , and the higher up-regulated expression of E sAB I1 compared with wide type. [Conclusion] EsABI1 acted as a negative regulator in ABA signal transduction pathway of E. sa lsu g in eum ,wh ich participated in the re-sponse of E. salsugineum to environmental stress.

关 键 词：盐芥 EsABI1 ABA 基因沉默 基因表达 

分 类 号：S188[农业科学—农业基础科学]