山东省各地市蜜蜂黑蜂王台病毒抽样检测分析  

The sampling inspection analysis of black queen cell virus in various cities of Shandong province

作　　者：李桂林 王颖[2] 马兰婷[2] 郭恒俊[1] 胥保华[2] 郭兴启[1] 

机构地区：[1]山东农业大学生命科学学院,泰安271018 [2]山东农业大学动物科技学院,泰安271018

出　　处：《中国蜂业》2017年第11期13-17,共5页Apiculture of China

基　　金：山东省现代产业技术体系创新团队专项基金(No.SDAIT-24-04)

摘　　要：蜜蜂黑蜂王台病是由蜜蜂黑蜂王台病毒(Black queen cell virus,BQCV)引起的蜜蜂疾病,目前在世界范围内均有分布。为检测山东省蜜蜂黑蜂王台病毒在蜂群中的携带情况,本研究对山东省17地市部分蜂场蜜蜂携带BQCV的情况进行抽样调查,并首次通过逆转录聚合酶链式反应(RT-PCR)方法初步对17地市的蜜蜂样本进行了快速病原学检测。检测结果显示,山东省17地市的62个蜂场中有来自7个地市的13个蜂场的蜂群携带BQCV,检出率为4.769%。结合调研情况及鉴定结果,我们还提供了5条可能防御蜜蜂黑蜂王台病发生的措施。希望本研究为有效预防山东省蜜蜂黑蜂王台病的发生提供参考。Black queen cell disease is one of the honeybee diseases that is caused by black queen cell virus （BQCV）, which is spread around the world at present. In this paper, some apiaries of seventeen cities in Shandong province was chosen to perform sampling inspection analysis to detect the carrying situation of BQCV in the honeybee of Shandong province, and the selected samples were carried out preliminary rapid pathogen detection by reverse transcription and polymerase chain reaction （RT-PCR） for the first time. The results showed that the honeybee from thirteen apiaries of seven cities was detected carrying BQCV in sixty-two apiaries of seventeen cities in Shandong province. The detection rate was 4.769%. Furthermore, we provide five possible measures to prevent black queen cell disease in this study through combining investigation situation and detection result. We hope that the findings in this paper can provide a reference for the effective prevention of black queen cell disease in Shandong province.

关 键 词：蜜蜂黑蜂王台病毒 RT-PCR 山东省 

分 类 号：S895[农业科学—特种经济动物饲养]