无毒性单磷酸脂质A对小鼠血清集落刺激因子的影响  

EFFECTS OF NONTOXIC MONOPHOSPHORYL LIPID A ON COLONY STIMULATING FACTOR IN THE SERUM OF MICE

作　　者：肖锦松[1] 崔风军[1] 赵文仲[1] 

机构地区：[1]第二军医大学放射医学研究室,上海200433

出　　处：《中国药理学通报》1991年第2期149-152,共4页Chinese Pharmacological Bulletin

摘　　要：用~3H-TdR掺入法和琼脂集落培养法比较观察条件培养液(CM)中集落刺激因子(CSF)的活性,结果表明两法显著相关,从Re突变型的明苏尼达沙门氏菌Re_(595)(Salmonella Minnesota Re_(595))得来的单磷酸脂质A(MPLA)静注后小鼠血清CSF水平明显提高,12h达高峰,24h恢复正常,有明显的量效关系,在诱生CSF的同时还产生一些不耐热的集落抑制因子(CIF),说明MPLA是一种很强的CSF诱生剂。The activity of colony stimulating factor ( CSF) in the conditioned medium ( CM ) was studied with combination method of 3H-TdR incorporation assay and agar colony assay. These two assays were demonstrated to be replaceable each other. The mice were administered with nontoxic monophosphoryl lipid A(MPLA) which was derived from a Re mutant of Salmonnella Minnesota Re595. The results showed a significant elevation of CSF in the serum and reaching the top at 12th h and returning to normal by 24th h. There is a significant dose-resoonse relationship. The CSF was induced with accompany of formation of colony inhibiting factor (GIF), some of which were heat sensitive factors. It is suggested that the MPLA may be a potent CSF-inducer.

关 键 词：单磷酸脂质A 免疫增强剂 

分 类 号：R979.5[医药卫生—药品]