钇-恩诺沙星增敏体系荧光光谱法测定饲料中恩诺沙星的含量  

Determination of trace enrofloxacin in feed with Y-enrofloxacin sensitized complex by fluorescence spectra

作　　者：李军德[1] 马池方 张婷婷 宋吉英[2] Li Junde Ma Chifang Zhang Tingting Song Jiying

机构地区：[1]青岛农业大学校医院,山东青岛266109 [2]青岛农业大学化学与药学院,山东青岛266109

出　　处：《饲料工业》2017年第19期55-60,共6页Feed Industry

摘　　要：恩诺沙星作为氟喹诺酮类化学合成抑菌剂经常作为兽用药与饲料混用。文章采用分子荧光光谱法,分析检测市售饲料中恩诺沙星的含量。实验主要研究了钇离子对恩诺沙星荧光强度的增敏作用,探讨了溶液的pH值,钇离子浓度,反应时间和反应温度等因素对钇-恩诺沙星溶液体系荧光强度的影响。结果表明,在最佳实验条件EX:277 nm,EM:441 nm,p H=6,Y^(3+)溶液浓度0.02 g/l,反应时间30 min,室温状态下进行分析测定,钇离子对恩诺沙星的荧光强度有非常明显的增敏效果。该方法在0~0.25 mg/l的浓度范围内线性良好,其线性相关系数R2为0.998 9,方法的检测限为1.02μg/l,平均加标回收率为103.1%。该方法用于测定饲料中痕量恩诺沙星的含量,稳定可靠,分析结果良好。Enrofloxacin is a bactericidal drug, and is often added into feed to kill active fungi in ani- mal tissues. In this article, a new method was developed for the determination of enrofloxacin in feed by fuorescence spectra, The sensitized effects of various factors, such as pH value, concentration of yt- trium ions, reaction time, reaction temperature on fluorescence were discussed and optimized. The flu- orescence was detected in excitation wavelength （277 nm） and emission wavelength （441 nm）. Under the optimal conditions, the fluorescence intensity of the system showed a good linear relationship with the concentration of enrofloxacin in the range of 0-0.25 mg/1.The linearly dependent coefficient was 0.998 9 and spiking avenge recovery was 103.1%. This method was simple, quick, accurate and effective for determination of enrofloxacin in feed. It was suitable for the quality control for enrofloxacin.

关 键 词：恩诺沙星 钇离子 荧光强度 增敏作用 

分 类 号：S816.17[农业科学—饲料科学]