情志刺激和内源性硫化氢与动脉粥样硬化发病的相关实验研究  

Role of Emotional Stimulation and Endogenous Hydrogen Sulfide in the Development of Atherosclerosis

作　　者：樊华 王秀英 赵志龙 林鹏 孙丹 王丽娜 康强 程嘉艺[1] 李雅君[1] Fan Hua Wang Xiuying Zhao Zhilong Lin Peng Sun Dan Wang Lina Kang Qiang Cheng Jiayi Li Yajun(Liaoning University of Traditional Chinese Medicine, Shenyang 110033, China Liaoning Institute for Drag Control)

机构地区：[1]辽宁中医药大学,沈阳110033 [2]辽宁省药品检验检测院

出　　处：《中国药师》2017年第9期1538-1542,共5页China Pharmacist

基　　金：辽宁省科学事业公益研究基金(编号:2015005006)

摘　　要：目的:观察情志刺激对动脉粥样硬化(AS)形成过程的影响,阐述硫化氢(H_2S)在动脉粥样硬化病变中的作用。方法:24只SD大鼠随机分为AS组、气滞血瘀型AS组和正常对照组,AS组给予特殊饲料,气滞血瘀型AS组给予特殊饲料+情志刺激,正常对照组给予普通饲料。造模期间,分别在第4、第8、第12周测定大鼠表征评分、H_2S含量、凝血四项、组织血流量、血脂指标。结果:气滞血瘀型AS组与AS组相比,第4周开始大鼠表征出现活动量明显减少、反应迟钝、被毛无光泽、舌质紫黯等现象(P<0.01);血脂明显升高,并随时间的增长而升高(P<0.05或P<0.01);第8周开始活化部分凝血活酶时间(APTT)、纤维蛋白原(FIB)明显升高(P<0.05);第8周开始表皮、肝脏、肾脏组织血流量均明显减少;第12周时H_2S的含量明显升高(P<0.01)。结论:情志刺激促进了AS模型的形成,同时气体分子H_2S体系在气滞血瘀型AS动物模型中也发挥了调节作用。Objective： To observe the effect of emotional stimulation on the formation process of atherosclerosis （AS）, and explain the role of hydrogen sulfide （H2S） in atherosclerotic lesions. Methods： Twenty-four Spragne-Dawley rats were randomly divided into AS group, qi-stagnation and blood-stasis AS group and the control group. The AS group was fed with special diets, the qi-staguation and blood-stasis AS group was fed with special diets and emotional stimulation, and the control group was fed with normal diets. During the experiment, the indicators including the characterization score, H2S content, four items of the natural bleeding and blood coagulation, tissue blood flow and blood lipid were respectively detected in the 4 th, 8 th and 12 th week. Results： Compared with those in the AS group, since the 4 th week, the rats in qi-stagnation and blood-stasis AS group were with siguificantly decreased activity, slow response, lackluster fur and dark purple tongue （P 〈 0.01 ） ; the level of plasma lipid increased significantly, and increased further with time extension（P 〈0.05 or P 〈0.01 ） ; since the 8 th week, APTT and FIB changed significantly （P 〈0.05） ,the blood flow to skin, liver and kidney decreased significantly （P 〈0.05） ; the content of H2S was significantly higher in the 12 th week （P 〈0.01 ）. Conclusion： Emotional stimulation promotes the formation of AS model, and the gas molecule system of H2 S plays a regulatory role in the qi-stagnation and blood-stasis AS animal model.

关 键 词：情志刺激 动脉粥样硬化 气滞血瘀型动脉粥样硬化 硫化氢 

分 类 号：R285.5[医药卫生—中药学]