复方三黄酊HPLC指纹图谱的建立和化学成分的归属  

Establishment of HPLC Fingerprint and Classification of Chemical Components for Compound Sanhuang Tincture

作　　者：丁长玲[1] 崔俊凤 张学美 傅勇[1] 樊其忠[1] 成文娜[1] Ding Changling Cui Junfeng Zhang Xuemei Fu Yong Fan Qizhong Cheng Wenna(Binzhou Medical University Hospital, Shandong Binzhou 256600, China Binzhou Center for Food and Drug Control Binzhou Maternal and Child Health Care Hospital)

机构地区：[1]滨州医学院附属医院,山东滨州256600 [2]滨州市食品药品检验检测中心 [3]滨州市妇幼保健院

出　　处：《中国药师》2017年第9期1564-1566,1572,共4页China Pharmacist

基　　金：滨州市科技计划课题项目(编号:2014ZC0146)

摘　　要：目的:采用高效液相色谱技术建立一种抗感染药物复方三黄酊乙醇部位的HPLC指纹图谱,同时分析其化学成分的归属。方法:色谱柱:Phenomenex Luna C_(18)(2)(250 mm×4.6 mm,5μm);流动相:乙腈-0.05 mol·L^(-1)磷酸二氢钾水溶液(22∶78);流速:1.0 ml·min^(-1);检测器:DAD检测器;检测波长:237 nm;柱温:30℃;进样量:10μl。结果:得到分离度和理论塔板数较好的复方三黄酊药效部位的指纹图谱,共标定出6个指纹特征峰,方法的重现性、稳定性和精密度良好;同时结合复方药物、单味药物和对照品的保留时间信息,确定了复方三黄酊中药效部位的特征峰来源。结论:所建立的抗感染药物复方三黄酊乙醇部位指纹图谱特征性强、重现性较好,对复方三黄酊乙醇部位的质量评价有重要的参考价值。Objective： To establish the HPLC fingerprint of ethanol parts of compound Sanhuang tincture （ an anti-infective drug） using high performance liquid chromatography, and analyze the classification of the chemical components. Methods： A Phenomenex Luna C18 （2） （250 mm ×4.6 mm, 5 μm） column was used. The mobile phase was acetonitrile-0.05 mol · L^-1 aqueous potassium dihydrogen phosphate solution （22 ： 78） and the flow rate was 1.0 ml · min^-1. The DAD detector was used and the detection wavelength was 237 nm. The column temperature was 30 ℃ and the injection volume was 10 μl. Results： The fingerprints of compound Sanhuang tincture were obtained with promising separation degree and the number of theoretical plates. A total of six fingerprint characteristic peaks were identified, and the reproducibility, stability and precision of the method were good. Meanwhile, combined with the information of retention time of compound medicine, single drug and reference substance, the source of characteristic peak of the effective part of compound Sanhuang tincture was determined. Conclusion： The fingerprints of compound Sanhuang tincture have strong characteristics and good reproducibility, which have important reference value for the quality evaluation of ethanol parts of compound Sanhuang tincture.

关 键 词：抗感染药物 复方三黄酊 高效液相色谱法 指纹图谱 化学成分归属 

分 类 号：R284.1[医药卫生—中药学] TQ460.72[医药卫生—中医学]