HPLC法测定柏石水调散中盐酸巴马汀和盐酸小檗碱的含量  

Determination of Palmatine Hydrochloride and Berberine Hydrochloride in Baishi Shuitiao Powder by HPLC

作　　者：黎春彤[1] 李翔[1] 马建丽[1] 张美芳[1] Li Chuntong Li Xiang Ma Jianli Zhang Meifang(Department of Pharmacy, the First Affiliated Hospital of PLA General Hospital, Beijing 100048, China)

机构地区：[1]解放军总医院第一附属医院药剂药理科,北京100048

出　　处：《中国药师》2017年第9期1668-1670,共3页China Pharmacist

基　　金：军队医疗机构制剂标准提高科研专项课题(编号:14ZJZ20-3)

摘　　要：目的:建立HPLC方法同时测定柏石水调散中盐酸巴马汀和盐酸小檗碱含量。方法:采用反相离子对高效液相色谱法,色谱柱为Agilent Zorbax SB-C_(18)(150 mm×4.6 mm,5μm);流动相为乙腈-0.1%磷酸溶液(50∶50)(每100 ml加十二烷基硫酸钠0.2 g);流速为1.0 ml·min^(-1);检测波长为345 nm;进样量为10μl;柱温为室温。结果:盐酸巴马汀和盐酸小檗碱进样量分别在1.11~22.16μg·ml^(-1)(r=0.999 6)和2.11~42.24μg·ml^(-1)(r=0.999 8)范围内与峰面积线性关系良好,平均回收率分别为98.94%和101.3%,RSD分别为2.43%和2.05%(n=6)。结论:本方法快速、简便、准确,可用于柏石水调散中盐酸巴马汀和盐酸小檗碱的含量测定。Objective： To establish an HPLC method for the determination of palmatine hydrochloride and berberine hydrochloride in Baishi Shuitiao Powder simultaneously. Methods： A reversed phase ion pair HPLC method was adopted. An Agilent Zorbax SB-C18 （ 150 mm ×4.6 mm,5μm） column was used, the mobile phase consisted of acetonitrile-0.1% phosphoric acid （50 ： 50） （0.2 g dodecyl sodium sulfate added to 100 ml solution）, the flow rate was 1.0 ml ·min^-1, the detection wavelength was at 345 nm, the injection volume was 10μl, the column temperature was at room temperature. Results： A good linear correlation was observed within the range of 1.11-22.16μg·ml^-1for palmatine hydrochloride （ r = 0. 999 6） and 2.11-42.24 μg·ml^-1 for berberine hydrochloride （ r = O. 999 8）. The average recovery was 98.94% with RSD of ：2.43% for palmatine hydrochloride and 101.3% with RSD of 2.05% for berberine hydrochloride （ n = 6）. Conclusion ： The method is rapid, simple and accurate, and can be used for the content determination of palmatine hydrochloride and berberine hydrochloride in Baishi Shuitiao Powder.

关 键 词：柏石水调散 盐酸巴马汀 盐酸小檗碱 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]