HPLC法同时测定骨筋丸胶囊中芍药苷、延胡索乙素和蛇床子素的含量  

Simultaneous Determination of Paeoniflorin,Tetrahydropalmatine and Osthole in Gujinwan Capsules by HPLC

作　　者：金情政[1] 郑明 Jin Qingzheng Zheng Ming(Zhejiang Quhua Hospital, Zhejiang Quzhou 324004, China Quzhou Institute for Food and Drug Control)

机构地区：[1]浙江衢化医院,浙江衢州324004 [2]衢州市食品药品检验研究院

出　　处：《中国药师》2017年第9期1694-1696,共3页China Pharmacist

摘　　要：目的:建立HPLC法同时测定骨筋丸胶囊中芍药苷、延胡索乙素和蛇床子素含量。方法:采用Eclipse XDB-C_(18)(250mm×4.6 mm,5μm)色谱柱,以0.1%磷酸溶液(三乙胺调节pH至6.6)-乙腈为流动相进行梯度洗脱,流速为1.0 ml·min^(-1),柱温为30℃,检测波长为230 nm和280 nm。结果:芍药苷、延胡索乙素和蛇床子素依次在9.56~33.65(r=0.999 8),3.65~12.86(r=0.999 9)和5.81~20.45μg·ml^(-1)(r=1.000 0)浓度范围内与峰面积呈良好线性关系,平均加样回收率分别为98.8%(RSD=1.1%),98.4%(RSD=0.8%)和99.1%(RSD=1.4%)(n=6)。结论:本方法简便、准确、重复性好,可用于该制剂的质量控制。Objective： To develop an HPLC method for the content determination of paeoniflorin, tetrahydropalmatine and osthole in Gujinwan capsules. Methods： The determination was performed on an Eclipse XDB-C18 column （250 mm ×4.6 mm,5μm） with the mobile phase consisting of 0.1% phosphoric acid （ adjusting pH to 6.6 with triethylamine） -acetonitrile with gradient elution. The flow rate was 1.0 ml ·min^-1. The column temperature was 30 ℃. The detection wavelengths were set at 230 nm and 280 nm. Re- sults ： The calibration curves were found to be linear within the range of 9.56-33.65μg·ml^-1 for paeoniflorin （ r = 0. 999 8 ）, 3.65- 12.86 μg·ml^-1 for tetrahydropalmatine （ r = 0.999 9） and 5.81-20.45μg·ml^-1 for osthole （ r = 1. 000 0）. The average recovery of paeoniflorin, tetrahydropalmatine and ostho/e was 98.8 % （RSD = 1.1% ）, 98.4 % （ RSD = 0.8 % ） and 99.1% （ RSD = 1.4% ） （ n = 6）, respectively. Conclusion： The method is simple, accurate and reproducible, which can be used for the quality control of Gujinwan capsules.

关 键 词：骨筋丸胶囊 芍药苷 延胡索乙素 蛇床子素 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]