诱导小鼠脾细胞合成NO的疟原虫抗原物质活性分析  

Experimental Study on Activity of Plasmodium Antigen to Induce Splenocytes of Mice to Produce NO

作　　者：阎建忠[1] 曹雅明[1] 刘英杰[1] 蔡天德[2] 

机构地区：[1]中国医科大学免疫学教研室,沈阳110001 [2]辽宁省疾病预防控制中心

出　　处：《中国公共卫生》2002年第9期1049-1050,共2页Chinese Journal of Public Health

摘　　要：目的　探讨疟原虫诱导小鼠脾细胞合成NO的抗原物质的性质。方法　应用Griess方法 ,分别检测加热处理的约氏疟原虫抗原以及经磷脂酶C、磷脂酶D和胰蛋白酶预处理的约氏疟原虫抗原对受染小鼠脾细胞合成NO的影响。结果　加热处理的约氏疟原虫抗原以及经磷脂酶C、磷脂酶D和胰蛋白酶预处理的约氏疟原虫抗原诱导脾细胞合成NO的水平 ,与对照组相比 ,没有显著差异。结论　诱导受染小鼠脾细胞合成NO的疟原虫抗原是一种耐热抗原 ,这种抗原成分中 (至少在功能区 )不含精氨酸和赖氨酸 ;Objective To clarify the activity of plasmodium antigen to induce splenocytes of mice to produce NO.Methods With Griess reaction,the effects of P.yoelii antigen treated with different factors on NO secretion from supernatants of cultured splenocytes were observed.Results The ability of P.yoelii antigen to induce NO synthesis by splenocytes is the same as that of P.yoelii antigen treated by heating as well as by phospholipase C.phospholipase D.trypsin.Conclusion The P.yoelii antigen is a heat-resisting antigen,being origin responsible for NO production in splenocytes,not containing lysine and arginine;GPI regarded as a major antigen of Plasmodium to induce splenocytes of mice to produce NO needs further study.

关 键 词：一氧化氮 疟原虫抗原物质 合成 活性分析 小鼠 脾细胞 

分 类 号：R382.31[医药卫生—医学寄生虫学]