湖北地区丙型肝炎病毒基因型分布及流行病学特征  

The distribution and epidemiological features of hepatitis C virus genotypes in Hubei Province

作　　者：宋娟 杨东亮[2] 孙自镛[3] 彭静[3] SONG Juan;YANG Dong-liang;SUN Zi-yong;PENG Jing(Department of Gastroenterology, Ninth Hospital of Wuhan, Wuhan, Hubei 430081 , China)

机构地区：[1]武汉市第九医院消化内科,湖北武汉430081 [2]华中科技大学同济医学院附属协和医院感染性疾病科 [3]华中科技大学同济医学院附属同济医院检验科

出　　处：《中国预防医学杂志》2017年第10期721-726,共6页Chinese Preventive Medicine

基　　金：国家科技重大专项项目(2012ZX10004207-004)

摘　　要：目的分析湖北地区丙型肝炎病毒(HCV)基因型分布及其流行病学特征。方法收集2013年5月至2016年3月在华中科技大学同济医学院附属同济医院接受HCV基因分型检查的丙肝患者血清及相关流行病学资料。采用实时荧光定量PCR对HCV RNA进行测定;采用巢式RT-PCR对HCV RNA阳性样本的核心区(core-e1)进行扩增并测序;对所测序列进行基因分型和进化树分析,并对各基因型相关的危险因素进行综合分析。结果共有HCV RNA阳性310例,测序223例,测序结果显示湖北地区存在5种基因型,1b型178例(79.82%),2a型32例(14.35%),6a型8例(3.59%),3b型4例(1.79%),3a型1例(0.45%)。1b、2a型主要见于1997年以前的有偿供血者和输血者;2a还多见于通过性接触传播的人群;6a、3b和3a型仅见于静脉注射毒品者。结论湖北地区HCV基因型分布呈现多样性且与传播途径密切相关,有关各方应予以关注及干预。Objective To understand the distribution and epidemiological features of hepatitis C virus（HCV）genotypes in Hubei.Methods Patients with hepatitis C were recruited from Tongji Hospital of Tongji Medical College,Huazhong University of Science and Technology in Hubei from May 2013 to March 2016,who were tested for HCV genotypes in the Department of Clinical Laboratory.Their blood samples and related epidemiological data were collected.Real-time PCR was used to quantify HCV RNA and nested RT-PCR was applied to amplify the core-e1 region of the HCV genome of HCV RNA-positive samples and PCR products were sequenced.HCV genotypes were determined by phylogenetic analysis and their corresponding risk factors were analyzed.Results A total of 310 samples were HCV RNA positive and 223 PCR products were sequenced.Five genotypes were identified with 79.82%（178/223）of 1b,14.35%（32/223） of 2a,3.59%（8/223） of 6a,1.79%（4/223）of 3b and 0.45%（1/223）of 3a.Analysis of genotype-associated risk factors revealed that unsafe paid blood donation and transfusion before 1997 were strongly associated with genotype 1b and 2a,the later was also related to sexual contact.Genotype 6a,3b and 3a were only found in intravenous drug users.Conclusions The distribution of HCV genotypes is diverse and highly associated with transmission routes in Hubei Province.Attention should be paid and intervention should be taken to control HCV infection.

关 键 词：丙型肝炎病毒 基因型 流行病学 系统进化树分析 地域分布 

分 类 号：R373.21[医药卫生—病原生物学]