重组IL-4R蛋白对SD大鼠过敏性哮喘干预作用的研究  

Intervention of allergic asthma with recombinant IL-4R in rats

作　　者：杨光勇[1] 郭海涛[1] 刘茜明 刘莉莉 何光志[1] YANG Guangyong GUO Haitao LIU Xirning et al(Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Chin)

机构地区：[1]贵阳中医学院基础医学院,550025

出　　处：《浙江医学》2017年第17期1417-1420,共4页Zhejiang Medical Journal

基　　金：2011年贵州省社会发展科技攻关项目[黔科合SY字(2011)3020];贵州省委组织部高层次人才科研条件特助经费(TZJF-2011年28号)

摘　　要：目的探索重组IL-4R蛋白对过敏性哮喘动物模型的干预作用。方法选择6周龄SD大鼠60只,采用安全随机分组法分成哮喘模型组、IL-4R组、布地奈德组和对照组,每组15只。哮喘模型组在腹部选取3处于皮下注射10%鸡卵清白蛋白(OVA)混合液1ml(OVA 100mg、氢氧化铝100mg及灭活的百日咳杆菌5×109个)进行致敏,1周后用上述同样药物再进行致敏,第2次致敏1周后将大鼠分别放入不完全封闭的箱中,用5%OVA与0.9%氯化钠混合溶液,连续10d超声雾化激发30min。IL-4R组和布地奈德组同法造模,但每次雾化激发前30min,IL-4R组腹腔注射重组IL-4R蛋白稀释液(400μg/d)。布地奈德组腹腔注射布地奈德稀释液(400μg/d),对照组用0.9%氯化钠溶液连续10d超声雾化激发30min。取4组大鼠肺组织作HE染色的光镜病理切片,观察肺组织病理变化。建立间接ELISA方法,检测实验中4组大鼠肺泡灌洗液中的IL-4和IFN-γ的含量。结果哮喘模型组大鼠肺组织有大量嗜酸性粒细胞浸润,对照组大鼠肺组织几乎无嗜酸性粒细胞浸润,IL-4R组和布地奈德组大鼠肺组织嗜酸性粒细胞浸润明显少于哮喘模型组。IL-4R组和布地奈德组肺泡灌洗液中IL-4和IFN-γ的含量明显高于哮喘模型组,差异均有统计学意义(均P<0.05)。结论研究结果提示IL-4R蛋白对过敏性哮喘有良好的干预作用。Objective To investigate the effect of recombinant IL-4R on allergic asthma in rats.Methods Sixty SD rats in 6 wk of age were randomly divided into model group,IL-4R group,budesonide group （as positive control） and control group with 15 in each.Asthma model was induced with subcutaneous injection of ovalbumin （OVA）.Rats in IL-4R group,budesonide group and control group were given intraperitoneal injection of recombinant IL-4R （400μg/d）,budesonide （400lμg/d） or normal saline,respectively.The pathological changes of lung tissues were observed with HE staining,the contents of IL-4 and IFN-γ in alveolar lavage fluid were detected by indirect ELISA method.Results Large amount of eosinophil infiltration in the lung tissue was observed in asthma model group,which were significantly attenuated in IL-4R budesonide groups.The contents of IL-4 and IFN-γ in alveolar lavagefluid of IL-4R and budesonide groups were significantly lower than those of asthma model group （P〈0.05）.Conclusion The results suggest that recombinant IL-4R can effectively intervene allergic asthma in rats.

关 键 词：IL-4R IFN-Γ ELISA 过敏性哮喘 

分 类 号：R562.25[医药卫生—呼吸系统]