全细胞膜片钳研究离体小鼠内侧膝状体亚核神经元电生理特性  

In Vitro Whole-cell Patch Clamp Recordings of Neurons in Subnuclei of Mouse Medial Geniculate Body

作　　者：杜小凤 贾慧娟 王欣[1] DU Xiao-feng JIA Hui-juan WANG Xin(Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University Wuhan 430079, Hubei, Chin)

机构地区：[1]华中师范大学生命科学学院遗传调控与整合生物学湖北省重点实验室,中国湖北武汉430079

出　　处：《生命科学研究》2017年第5期386-391,共6页Life Science Research

基　　金：华中师范大学基本科研业务费青年教师项目(CCNU15A05035)

摘　　要：内侧膝状体(medial geniculate body,MGB)是听觉通路在丘脑的重要中继站,为了探究小鼠MGB亚核神经元的电生理特性,使用离体全细胞膜片钳技术记录到99个MGB神经元,其中腹侧核(ventral division of MGB,MGBv)神经元36个,背侧核(dorsal division of MGB,MGBd)神经元34个,内侧核(medial division of MGB,MGBm)神经元29个。结果表明,神经元对去极化电流的反应具有爆发型(bursting)(16.2%,16/99)和紧张型(tonic)(83.8%,83/99)两种发放模式,并且所有MGB神经元在超极化电流刺激结束后均有去极化反弹(rebound),而仅有少数神经元(17.2%,17/99)在超极化刺激过程中有超极化激活的内向电流(hyperpolarization-activated inward current,I_h)。此外,MGB各亚核神经元的静息膜电位(resting membrane potential,RMP)和激活阈值(active threshold)存在显著性差异(P<0.05)。以上表明MGB各亚核的电生理特性存在差异,该结果有助于理解MGB各亚核在听觉信息处理中产生不同功能的原因。Medial geniculate body （MGB） is an important thalamic relay along the auditory pathway. To inves- tigate the electrophysiological properties of MGB neurons, 99 MGB neurons were obtained by using in vitro whole-cell patch clamp recording technique, including 36 neurons from the ventral division of the MGB （MGBv）, 34 neurons from the dorsal division of the MGB （MGBd） and 29 neurons from the medial division of the MGB （MGBm）. Results showed that two firing patterns including bursting （16.2%, 16/99） and tonic （83.8%, 83/ 99） were identified based on the responses to depolarizing current, and that depolarizing rebounds were found at the offset of hyperpolarizing currents in all of the MGB neurons. Only some of the neurons （17.2%, 17/99） also showed hyperpolarization-activated inward current （Ih） to hyperpolarizing currents. Moreover, the resting membrane potential （RMP） and active threshold showed significant differences among neurons of dif- ferent subnuelei （P〈0.05）. These results indicate that MGB subnuclear neurons are different in electrophysi- ologieal properties. This would help us understand the distinct functions of MGB neurons in central auditory processing.

关 键 词：小鼠 全细胞膜片钳 内侧膝状体(MGB) 发放模式 静息膜电位(RMP) 激活阈值 

分 类 号：Q437[生物学—生理学]