拟南芥LecRK-ConA基因突变体的筛选鉴定及其功能初步研究  

The Mutant Identification and Preliminary Functional Study of LecRK-ConA Gene in Arabidopsis thaliana

作　　者：何壮 李金燕 张骋 肖文君 刘书灿 李美玲 郭新红 HE Zhuang LI Jin-yan ZHANG Cheng XIAO Wen-jun LIU Shu-can LI Mei-Ling GUO Xin-hong(Hunan Provincial Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410082, Hunan, China)

机构地区：[1]湖南大学生物学院植物功能基因组学与发育调控湖南省重点实验室,中国湖南长沙410082

出　　处：《生命科学研究》2017年第5期399-404,共6页Life Science Research

基　　金：国家自然科学基金资助项目(31540064,31071076,30871325);教育部高等学校博士学科点专项科研基金(20130161110005);植物分子遗传国家重点实验室资助项目(2015);湖南省研究生科研创新项目(CX2017B145,CX2015B073,CX2016B097);湖南省重点研发计划项目(2016WK2003);国家重点研发计划项目子课题(2017YFF0210301)

摘　　要：植物凝集素类受体激酶(lectin receptor-like kinases,LecRLKs)在植物抗逆性、激素信号转导及生长发育调节等方面扮演重要角色。LecRK-ConA基因是拟南芥Lec RLK基因家族中的一员。利用三引物法鉴定获得LecRK-ConA基因两个缺失突变体的纯合子。启动子顺式作用元件分析显示,LecRK-ConA具有多个与干旱、光、赤霉素(gibberellic acid,GA)等应答相关的元件。实时定量PCR结果表明:LecRK-ConA基因具有组织表达差异性,且在春化后4 d的种子中表达量最强,用NaCl和甘露醇处理均能抑制该基因的表达。用NaCl和甘露醇处理缺失突变体lecrk-cona和Col-0后,发现lecrk-cona的种子萌发率显著低于野生型。此外,突变体lecrkcona相对野生型Col-0来说,具有早花现象。上述实验结果为进一步深入研究LecRK-ConA基因在抗逆与开花调控途径中的生理功能奠定了基础。Lectin receptor-like kinases （LecRLKs） play an important role in plant resistance, hormone signal pathways and plant growth. The LecRK-ConA gene is a member of the Arabidopsis LecRLK family. Here, three primer PCR methods were used to identify the homozygous T-DNA mutants of LeeRK-ConA gene. The analysis of cis-elements in promoter showed that LecRK-ConA gene had multiple drought, light and GA responsive elements. The results of real-time quantitative PCR showed that the expression of LecRK-ConA gene had tissue differences and it had the strongest expression in seeds after vernalization for four days. The expression of LecRK-ConA gene was inhibited under NaC1 and mannitol treatments in A rabidopsis. The seed germination rate of lecrk-cona was lower than that of the wild type under NaC1 and mannitol treatments. Furthermore, lecrk-cona mutants flowered early compared to Col-0 plants. The results provide a basis for further study on the function of LecRK-ConA gene involved in stress tolerance and flowering regulation.

关 键 词：拟南芥 LecRK-ConA基因 凝集素类受体激酶(LecRLKs) 逆境胁迫 开花调节 

分 类 号：Q785[生物学—分子生物学]