噬菌体治疗泛耐药鲍氏不动杆菌重症感染小鼠的研究  

The treatment of severe infection of mice caused by pandrug resistant Acinetobacter baumannii with bacteriophages

作　　者：闫李侠[1] 黄至澄[1] 任应鹏[1] 李恪梅 陈保文 王国治 

机构地区：[1]浙江省台州市第一人民医院检验科,台州318020 [2]中国药品生物制品检定研究院,北京100050

出　　处：《中国人兽共患病学报》2017年第10期920-922,共3页Chinese Journal of Zoonoses

摘　　要：目的探讨噬菌体治疗泛耐药鲍氏不动杆菌重症感染小鼠的疗效。方法以SPF级昆明小鼠建立小鼠感染泛耐药鲍氏不动杆菌的模型,分为实验组和对照组,实验组使用噬菌体AB46治疗,对照组给同等体积的肉汤治疗,统计比较两组小鼠的生存率,进行脾菌数分析。结果泛耐药鲍氏不动杆菌1∶10稀释实验组与对照组P=0.020<0.05,有统计学差异;1∶2、1∶5稀释组分别为P=0.650,P=0.170,均>0.05,无统计学差异;1∶50稀释的实验组与对照组均无小鼠死亡,脾菌数计数结果 P=0.026,有统计学差异。结论噬菌体治疗泛耐药鲍氏不动杆菌重症感染小鼠是一种有效的治疗方法,可明显提高小鼠的生存率,在感染较轻的情况下可以明显降低小鼠的脾菌数。We explored the effect of therapy with bacteriophages for pandrug resistant Acinetobacter baumannii infections.Kunming mice（Specific Pathogen Free）were divided into two groups：experimental group（severe infection caused by pandrug resistant Acinetobacter baumannii）and the control group.The mice of experimental group were treated with Phages AB46 while the mice of control group were treated with broth.The survival rate was compared with statistical method and the spleen bacteria count was analyzed.Results showed that there were statistical difference between the experimental group and control group of Pandrug resistant Acinetobacter baumannii groups of 1∶10 dilution,P=0.0200.05.There were no statistical difference between each two groups of 1∶2（P=0.650）and 1∶5（P=0.170）dilution,both were0.05.There were no dead mice in groups of 1∶50 dilution with statistical difference of spleen bacteria count,P=0.026.Therapy with phages was an effective method to control infection caused by Pandrug resistant Acinetobacter baumannii which could increase the survival rate and decrease spleen bacteria count of the mice with light infection.

关 键 词：噬菌体 治疗 鲍曼不动杆菌 感染 

分 类 号：R378[医药卫生—病原生物学]