HPLC测定脑得生固体分散体胶囊中葛根异黄酮类成分的含量  

Determination of Puerarin Isoflavones in Naodesheng Solid Dispersion Capsules by HPLC

作　　者：罗兰 李明丽[1] 康家珍 陈雨 王淑美 梁生旺[1,2,3] 

机构地区：[1]广东药科大学中药学院,广东广州510006 [2]广东药科大学国家中医药管理局中药数字化质量评价技术重点研究室,广东广州510006 [3]广东药科大学广东高校中药质量工程技术研究中心,广东广州510006

出　　处：《中华中医药学刊》2017年第11期2816-2818,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81274059)

摘　　要：目的:建立脑得生固体分散体胶囊中葛根异黄酮类成分(葛根素、大豆苷、大豆苷元、染料木素)的含量测定方法。方法:采用高效液相色谱法,Hyspersil ODS-2色谱柱(5μm,250 mm×4.6 mm),柱温25℃,流动相甲醇-水系统梯度洗脱,流速0.8 mL·min^(-1),检测波长250 nm。结果:葛根素在0.540 8~3.244 8μg(r=0.999 9)范围,大豆苷在0.130 0~0.780 0μg(r=0.999 9)范围,大豆苷元在0.073 2~0.439 2μg(r=0.999 9)范围,染料木素在0.044 8~0.268 8μg(r=0.999 9)范围均呈良好的线性关系;平均回收率分别为99.08%,98.10%,97.91%,99.26%;RSD分别为1.67%,1.57%,2.43%,2.92%。结论:所建立方法简便、准确、重复性好,可有效控制该制剂质量。Objective： To simultaneously determine the contents of puerarin, daidzin, daidzein and genistein in Naodesheng Solid Dispersion Capsules by HPLC. Methods： An external standard method by HPLC with Hyspersil ODS - 2 C18 column （5 μm, 250 mm ×4.6 mm） was used with the column temperature was 25 ℃. The detection wavelength was 250 nm. The flow rate was set at 0.8mL·min^-1 and gradient elution with methanol and H20. Results ： Linearities of puerarin, daidzin, daidzein and genistein were good in the ranges of 0. 540 8～ 3. 244 8μg （r = 0. 999 9）, 0.130 0 - 0. 780 0μg （ r = 0. 999 9）, 0. 073 2 - 0. 439 2μg （ r = 0. 999 9 ） and 0. 044 8 - 0. 268 8 μg （ r = 0. 999 9 ） , respectively. The average recoveries of puerarin, daidzin, daidzein and genistein were 99.08%, 98.10% , 97.91% and 99.26% and RSD values were 1.67% , 1.57% , 2.43% and 2.92% , respectively. Conclusion： The method is simple,accurate and reproducible, and can be used for quality control of Naodesheng Solid Dispersion Capsules.

关 键 词：脑得生固体分散体胶囊 HPLC 葛根素 大豆苷 大豆苷元 染料木素 

分 类 号：R284.1[医药卫生—中药学]