针刺对支气管哮喘大鼠肺组织c-fos蛋白表达的影响  

Effect of Acupuncture on Expression of c-fos Protein in Lung Tissue of Asthmatic Rats

作　　者：韩君萍 李双 赵叶 杨金华 虞跃跃 孙娜 崔建美 

机构地区：[1]华北理工大学中医学院,河北唐山063000

出　　处：《中华中医药学刊》2017年第11期2892-2895,I0012-I0014,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81303046)

摘　　要：目的:探讨针刺对支气管哮喘大鼠肺组织中c-fos蛋白表达的影响。方法:将40只SPF级SD大鼠随机分为空白组、哮喘模型组、哮喘模型针刺组、哮喘针刺对照组,每组10只。各组分别给予相应干预后,采用细胞计数、HE染色法观察大鼠肺组织中炎症程度及病理变化,免疫组织化学染色法检测肺组织中c-fos蛋白的表达程度,免疫荧光染色法检测肺组织中TGF-β1的表达。结果:哮喘针刺组大鼠细胞计数中白细胞及嗜酸性粒细胞较哮喘模型组数量减少但较空白组多(P<0.05);哮喘模型针刺组大鼠肺组织病理改变较哮喘模型组减轻但较空白组重;免疫组化及免疫荧光结果显示,哮喘针刺组大鼠肺组织c-fos蛋白表达(IOD值)低于哮喘模型组(P<0.05)。结论:针刺可明显改善支气管哮喘大鼠炎症反应,能明显抑制c-fos的蛋白表达,针刺可能通过抑制c-fos蛋白表达而达到减轻支气管哮喘症状的目的。说明原癌基因c-fos与支气管哮喘的发作有密切关系。Objective ： To examine the mechanism of acupuncture on c - fos expression in the lung tissues of asthmatic rats. Methods ： Forty SPF male SD rats were randomly divided into 4 groups with 10 rats in each group. Groups were giv- en accordingly interventions. Light microscope was used to observe the effects of acupuncture on c -fos expression with cell counting, HE staining, immunohistochemistry method and Immunofluorescence method. Results： Cell count of rats＇ lungs indicated that the number of leukocytes and eosinophils ceils of acupuncture group were less than that of the asthmatic model group,but were larger than those of blank group （ P 〈 0. 05 ）. HE staining of rats＇ lungs indicated that the pathological changes of acupuncture group were slighter than those of the asthmatic model group, but were worse than those of blank group. Immunohistochemistry showed that the protein expression of c - fos （IOD） in lung tissue in acu- puncture group were lower than that in the asthmatic model group （P 〈 0. 05 ）. Conclusion ： Acupuncture can effectively decrease the c - fos protein quantity of lung tissues, indicating the onset is closely related to proto - oncogene c - fos and bronchial asthma.

关 键 词：针刺 支气管哮喘 C-FOS 

分 类 号：R245.3[医药卫生—针灸推拿学] R256.12[医药卫生—中医临床基础]