黄芪液拮抗乳酸盐腹透液对人腹膜间皮细胞的损伤作用  

EFFECTS OF HUANGQI INJECTION ON THE CYTOTOXICITY OF LACTATE-BUFFERED PERITONEAL DIALYSIS SOLUTES TO HUMAN PERITONEAL MESOTHELIAL CELLS

作　　者：刘映红[1] 刘伏友[1] 段绍斌[1] 彭佑铭[1] 肖平[1] 李军[1] 卓莉[1] 袁芳[1] 樊敏[1] 

机构地区：[1]中南大学湘雅二医院肾科,长沙410011

出　　处：《中国现代医学杂志》2002年第16期32-33,39,共3页China Journal of Modern Medicine

基　　金：湖南省自然科学基金项目 (0 1JJY2 0 71);湖南省卫生厅基金项目 (2 0 0 1-Y -3 9)

摘　　要：目的 :研究黄芪液对乳酸盐腹透液致腹膜间皮细胞增殖及损伤作用的影响。方法 :采用胰蛋白酶 -ED TA消化法 ,从人腹膜组织中分离间皮细胞 ,并建立体外人腹膜间皮细胞 (HPMC)培养模型。以MTT法测定HPMC增殖程度 ,采用自动生化分析仪检测 ,培养液中乳酸脱氢酶 (LDH)水平示细胞损伤程度。结果 :2 .5 %乳酸盐腹透液中加用黄芪液 (1mg/ml)与HPMC作用 4 5min ,其培养液中LDH水平较腹透液对照组明显降低(P <0 .0 1) ,HPMC增殖能力明显增强 (P <0 .0 1)。结论 :黄芪液能拮抗乳酸盐腹透液致HPMC的损伤。Objective:To determine the effects of huangqi injection on the cytotoxicity of lactate-buffered peritoneal dialysis solutes(L-PDS) to human peritoneal mesothelial cells (HPMC).Methods:HPMC was separated with trypsin-EDTA.HPMC proliferation and the concentration of lactate dehydrogenase (LDH) in supernatants were detected by MTT assay and automatic biochemical analyzor,respectively.Results:The addition of 1mg/ml of huangqi to 2.5% L-PDS caused an increase in HPMC proliferation, and a decrease in release of LDH for exposure 45min( P <0.01).Conclusion:Huangqi injection can reverse the injury of L-PDS to HPMC.

关 键 词：腹膜透析 间皮细胞 乳酸盐类 黄芪注射液 乳酸酸腹透液 

分 类 号：R459.5[医药卫生—治疗学]