不同品系昆虫病原线虫对烟草小地老虎的致病力  

Evaluation of different strains entomopathogenic nematodes against Agrotis ypsilon Rottemberg (Lepidoptera,Noctuidae) in tobacco

作　　者：李根 许文君 王新中[2] 户艳霞[2] 冼继东[1] 

机构地区：[1]华南农业大学农学院,广州510642 [2]云南省烟草公司大理市公司,云南大理671000

出　　处：《环境昆虫学报》2017年第5期1025-1031,共7页Journal of Environmental Entomology

基　　金：中国烟草总公司云南省公司科技计划项目专项(2014YN29)

摘　　要：小地老虎Agrotis ypsilon Rottemberg是烟田的一种重要的地下害虫。为了探明不同品系的昆虫线虫对烟田地下害虫小地老虎的致病力,在室内采用生测试验法,以滤纸为介质,测定了斯氏属线虫Steinernema carpocapsae和异小杆属线虫Heterorhabditis bacteriophora共5个品系对小地老虎3龄幼虫、5龄幼虫和蛹的致病力。结果表明,5个不同昆虫病原线虫品系均对小地老虎3龄幼虫具有致病力,其中斯氏线虫S.carpocapsae-A11(Sc-A11)对小地老虎的致死速度最快,侵染24 h、36 h和72 h后小地老虎校正死亡率分别为41.3%、69.7%和86.7%,均高于其它线虫品系,小地老虎的死亡率随着线虫侵染时间的增加而增加的,至96 h后校正死亡率达到100%,Sc-A11侵染5龄幼虫的校正死亡率均高于其它线虫品系,可见Sc-A11对小地老虎幼虫的致病力最强,为最敏感的昆虫病原线虫品系。Sc-All对小地老虎3龄幼虫、5龄幼虫的校正死亡率在72 h时分别为86.7%、43.3%,化蛹率为23.7%,Sc-All不侵染小地老虎蛹。盆栽烟草上线虫Sc-A11剂量为20头IJs/cm^2施用3次和线虫剂量为80头IJs/cm^2施用2次的处理的保苗效果最好,3 d后保苗效果分别为79.64%和76.85%,达到最佳的防治效果。The black cutworm, Agrotis ipsilon Rottemberg （Lepidoptera, Noctuidae）, is one of the most important ground pests which seriously damage on tobacco. In present study, the pathogenicity of 5 species of entomopathogenic nematodes （EPNs） against 3rd instar larvae, 5th instar larvae and pupae of A. ipsilon were determined with Steinernema carpocapsae and Heterorhabditis bacteriophora in the laboratory, respectively. The results showed that 5 species of entomopathogenic nematodes （ EPNs ） significantly affected the survival rate of 3 rd instar larvae of A. ipsilon. The infection incubation of EPN S. carpocapsae-A11 （ Sc-A11 ） against 3 rd instar larvae of A. ipsilon was the highest. The corrected mortality rates of 3 rd instar larvae of A. ipsilon infected by Sc-A11 were 41. 3%, 69. 7% and 86. 7% after 24 h, 36 h and 72 h, respectively, which higher than other EPNs. The mortality rates of 3rd instar larvae of A. ipsilon increased with increase in infection incubation from 24 h to 72 h and corrected mortality rate was 100% after 96 h. The corrected mortality rates of 5 th instar larvae infected by Sc-All were higher than that infected by other ENPs. That was to say, The ENP Sc-All had the highest pathogenicity against larvae of A. ipsilon. The corrected mortality of 3 rd instar larvae and 5 th instar larvae were 86. 7% and 43. 3%, the pupation rate of A. ipsilon was 23. 7% which infected by Sc-All after 72 h, While pupae of A. ipsilon was not infected by EPN Sc-All. The pot experiment showed that protection efficiency for tobacco of Sc-A11 was the best which used 20 IJs/cm2 applied 3 times and 80 IJs/cm2 applied 2 times. The survival rates of tobacco were 79. 64% and 76. 85% after 3 d, which showed the best control efficiency.

关 键 词：小地老虎 昆虫病原线虫 生物测定 致病力 烟草 

分 类 号：Q968.1[生物学—昆虫学] S476[农业科学—农业昆虫与害虫防治]