茧蜂病毒调控寄主细胞的NF-κB信号通路  

Mb BV regulates host cell's NF-κB signal pathway

作　　者：徐沙 刘樾彤 罗开珺[1] 

机构地区：[1]云南大学生命科学学院云南省高校动物遗传多样性和进化重点实验室,昆明650091

出　　处：《环境昆虫学报》2017年第5期1090-1099,共10页Journal of Environmental Entomology

基　　金：国家自然科学基金项目(31060251;31260448;31471823);云南省应用基础研究计划重点项目(2013FA003)

摘　　要：多分DNA病毒(Polydnaviruses,PDVs)是寄生蜂的共生病毒。已有研究报道PDVs能感染寄主血细胞并有特异性表达,但是,哪些病毒的DNA片段能进入寄主细胞,并参与调控NF-κB信号通路仍然还不清楚。本研究结果初步表明,在双斑侧沟茧蜂病毒(Microplitis bicoloratus bracovirus,MbBV)感染细胞后24 h能导致细胞凋亡,vank86、vank92、ptp109病毒基因片段均能在被感染后的Spli221(斜纹夜蛾)、Sf9(草地贪夜蛾)、High Five(粉纹夜蛾)细胞的DNA中检测到,但转录水平有差异。进一步对这3个基因与NF-κB调控的下游抗菌肽基因(attacin、defensin)和免疫基因酚氧化酶原激活酶(ppA3)的相互关系研究发现:在病毒感染Spli221、Sf9、High Five细胞24 h后,检测到Spli221细胞中attacin、defensin、ppA3的转录本,attacin基因显著下调;过表达Vank86、Vank92、PTP109的Spli221细胞,由LPS刺激后检测到只表达单个病毒蛋白的Spli221细胞中的attacin、defensin、ppA3的m RNA表达水平无显著变化。研究初步揭示了MbBV病毒感染Spli221细胞后抑制NF-κB信号通路,但单个病毒蛋白不能抑制NF-κB信号通路,实验结果为进一步深入研究多个病毒基因的共同作用及其在害虫生物防治中的应用打下了基础。Polydnaviruses （ PDVs ） are symbiotic virus of parasitoid wasps. It has been reported that PDVs can infected and specifically express on the host hemocytes. However, which virus DNA segments can enter the host cell and participate in the regulation of NF-κB signal pathway remain unclear. Our preliminary research results showed that Microplitis bicoloratus bracovirus （ MbBV ） can induce cells apoptosis after infected 24 h. MbBV-vank86 , vank92 , ptp109 segments were detected in insect cells （Spli221, Sf9, High Five） after infected with MbBV, but there were differences on transcription levels. Further more, We explore the relationship between three genes and downstream of NF-κB signal pathway antibacterial peptide （ attacin, defensin ） and immune gene prophenolosidase-activating enzyme （ppA3）： When MbBV infected Spli221, Sf9, High Five 24 hours, we detect transcription level of attacin, defensin, ppA3. We find attacin down-regulation extremely. Vank86, Vank92, PTP109 over-expression were detected in Spli221, attacin, defensin, ppA3 mRNA level have no significant difference after LPS stimulated. These results suggested that MbBV can regulate NF-κB signal pathway in Spli221 cell, but single virul protein cann＆#39;t inhibit NF-κB signal pathway. The results of this study could offer a reliable experiment basis for exploringmultiple genes function together and insect pests biological control in the future.

关 键 词：多分DNA病毒 脂多糖 NF-ΚB 抗菌肽 酚氧化酶原激活酶 

分 类 号：Q963[生物学—昆虫学] S476[农业科学—农业昆虫与害虫防治]