老年心力衰竭大鼠心脏和肺β_3肾上腺素能受体的实验研究  

β_3-adrenoceptor in heart and lungs of elderly heart failure rats

作　　者：徐子博 白灵丽 陈哲[2] 

机构地区：[1]北京市中关村中学,100086 [2]首都医科大学附属北京朝阳医院内分泌科

出　　处：《中华老年心脑血管病杂志》2017年第11期1192-1195,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：基金项目:代谢紊乱相关心血管疾病北京市重点实验室(pxm2017_014226_000046);首都医科大学本科生科研创新(xsky2014090)

摘　　要：目的探讨老年心力衰竭(心衰)模型大鼠心脏及肺β3肾上腺素能受体(β3-AR)变化及相互关系。方法选择雄性老年Wistar大鼠48只,应用主动脉结扎术制备心衰模型。大鼠随机分为假手术组和心衰组,每组24只,每组术后5、7、9、11周每个时间点各6只。测定2组大鼠血流动力学、病理、心肌及肺组织β3-AR mRNA表达。结果与假手术组比较,心衰组术后9、11周心率、左心室收缩末压、左心室压力最大上升速率显著降低,左心室压力最大下降速率显著增高,5、7、9、11周左心室舒张末压显著增高,差异有统计学意义(P<0.01)。心衰组术后7周出现肺水肿,术后9周出现心肌核坏死。心衰组术后5、7、9、11周每个时间点肺组织β3-AR mRNA表达较2周前均明显减低(P<0.05)。心衰组术后9、11周心肌组织β3-AR mRNA表达较假手术组明显升高(1.21±0.26 vs 0.98±0.22,1.26±0.23 vs 1.05±0.24,P<0.01)。结论心衰时,肺组织β3-AR mRNA表达下降,心肌组织表达升高,且肺的变化先于心肌组织。Objective To study theβ3-adrenoceptor（β3-AR）in heart and lungs of elderly heart failure（HF）rats.Methods Forty-eight elderly HF Wistar rats were included in this study.A HF model of rats was established by ligating the aorta.The rats were divided into sham operation group（n=24）and HF group（n=24）.The rats in each group were further divided into 4 subgroups at weeks 5,7,9 and 11 after operation（6 in each group）.The hemodynamics,pathology and expression ofβ3-AR mRNA and protein in heart and lungs were detected at weeks 5,7,9 and11 respectively after operation.Results The heart rate,LVESP,and dp/dtmax were significantly lower in HF group than in sham operation group at weeks 9 and 11 after operation while the LVEDP was significantly higher in HF group than in sham operation group at weeks 5,7,9 and 11 after operation（P〈0.01）.Pulmonary edema occurred at week 7 after operation and myocardial necrosis was detected at week 9 after operation.The expression level ofβ3-AR mRNA in lungs was significantly lower in HF group at weeks 5,7,9 and 11 than at week 2 after operation（P〈0.05）.The expression level ofβ3-AR mRNA in heart was significantly higher in HF group than in sham operation group at weeks 9 and 11 after operation（1.21±0.26 vs 0.98±0.22,1.26±0.23 vs1.05±0.24,P〈0.01）.Conclusion Theβ3-AR mRNA expression is downregulated in the lungs and upregulated in the heart.

关 键 词：心力衰竭 受体 肾上腺素能β3 血流动力学 心室功能 左 

分 类 号：R541.6[医药卫生—心血管疾病]