正五聚素蛋白3在氧化型低密度脂蛋白诱导的平滑肌细胞中的表达  

Expression of PTX3 in smooth muscle cells induced by oxidized low density lipoprotein

作　　者：易立[1] 脱厚珍[1] 郭芳[1] 赵媛[1] 王琦 陈滢宇 

机构地区：[1]首都医科大学附属北京友谊医院神经内科,100050 [2]北京城市学院

出　　处：《中华老年心脑血管病杂志》2017年第11期1196-1198,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家自然科学基金(81400959)

摘　　要：目的通过体内和体外实验探讨正五聚素蛋白3(pentraxin 3,PTX3)与动脉粥样硬化的关系,以及在氧化型低密度脂蛋白(ox-LDL)诱导的平滑肌细胞增殖和迁移中的作用。方法选择雄性载脂蛋白E基因敲除小鼠20只,随机分为高脂饮食组和正常饮食组,每组10只。饲养16周后行主动脉超声检查,处死后取主动脉行苏木精-伊红染色及PTX3免疫组织化学测定。小鼠主动脉平滑肌细胞传代培养后,分别与不同浓度ox-LDL(0、20、50、100mg/L)共同孵育,选增殖最明显100mg/L ox-LDL作为实验组,并以正常主动脉平滑肌细胞作为对照组。检测平滑肌细胞增殖、迁移程度,PTX3蛋白表达水平。结果高脂饮食组动脉粥样硬化斑块程度较正常饮食组严重[(29.71±2.07)%vs(3.26±0.81)%,P<0.01]。实验组主动脉平滑肌细胞增殖、迁移和PTX3表达较对照组明显增高(P<0.05,P<0.01)。结论 PTX3参与了动脉粥样硬化的形成,并与脂质代谢相关。Objective To study the relationship between PTX3 and atherosclerosis,and the role of PTX3 in proliferation and migration of smooth muscle cells induced by oxidized low density lipoprotein（ox-LDL）.Methods Twenty male apolipoprotein E knockout mice were randomly divided into high-fat diet group（n=10）and normal diet group（n=10）.The animals underwent aortic ultrasonography after 16 weeks of feeding and were then killed to take aortic tissue samples which were stained with HE and PTX3 immunohistochemistry for detecting the proliferation and migration of smooth muscle cells induced by ox-LDL.The smooth muscle cells of aorta,coincubated with ox-LDL at the concentrations of 0 mg/L,20 mg/L,50 mg/L,100 mg/L and incubated with ox-LDL at the concentration of 100 mg/L after they were subclutured,served as an experimental group with normal smooth muscle cells of atorta served as a control group.The proliferation and migration of smooth muscle cells and PTX3 expression in smooth muscle cells were detected.Results The incidence of atherosclerotic plaques was significantly higher in high-fat diet group than in normal diet group（P〈0.01）.The proliferation and migration rate of smooth muscle cells and the PTX3 expression level in smooth muscle cells were significantly higher in experimental group than in control group（P〈0.05,P〈0.01）.Conclusion PTX3 is involved in the formation of atherosclerosis and is associated with lipid metabolism.

关 键 词：正五聚素蛋白3 脂蛋白类 LDL 动脉粥样硬化 载脂蛋白E类 小鼠 基因敲除 肌细胞 平滑肌 细胞增殖 

分 类 号：R543.5[医药卫生—心血管疾病]