结核感染中IL-23R mRNA表达水平对Th17/IL-17免疫应答的影响  

The effect of expression level of IL-23R mRNA on Th17/IL-17 immune response in mycobacterium tuberculosis infection

作　　者：金鎏 赵德胜 张衡 张治国[2] 曹树辉[3] 王伟[3] 金俊[4] 张晓楠[5] 苏虹[1] JIN Liu;ZHAO De-sheng;ZHANG Heng;ZHANG Zhi-guo;CAO Shu-hui;WANG Wei;JIN Jun;ZHANG Xiao-nan;SU Hong(Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei 230032, China;Office of the Vice President, Beijing Changping Center for Tuberculosis Control and Prevention, Belting 102200, China;Laboratory of Bacterial and Immunology, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China;Derpartmenrt of Blood Transfusion, Lu＇ an Municipke Hospital of Anhui Province, Lu＇ an 237010, China;Department of clinical Medicine, the First school of Clinical Medicine, Anhui Medical University, Hefei 230032, China)

机构地区：[1]安徽医科大学公共卫生学院流行病与卫生统计学系,安徽合肥230032 [2]北京市昌平区结核病防治所副所长办公室,北京102200 [3]首都医科大学附属北京胸科医院细菌免疫研究室,北京101149 [4]六安市立医院输血科,安徽六安237010 [5]安徽医科大学第一临床学院临床医学系,安徽合肥230032

出　　处：《中华疾病控制杂志》2017年第9期904-908,共5页Chinese Journal of Disease Control & Prevention

基　　金：安徽医科大学七年制临床医学专业"早期接触科研"训练计划立项制项目(2012-ZQKY-73)

摘　　要：目的分析白介素23受体(interleukin 23 receptor,IL-23R)对结核感染中辅助型T细胞17(T helper cell 17,Th17)免疫应答的影响,探讨IL-23R在肺结核(tuberculosis,TB)发病机制中的作用。方法选取2015年5~10月于北京胸科医院住院的21名活动性肺结核病人(active tuberculosis,ATB),2015年5~7月于北京昌平区结防所结核病体检的21名结核潜伏感染者(latent tuberculosis infection,LTBI)和21名健康对照(healthy donor,HD)为研究对象;分离外周血单个核细胞(peripheral blood mononuclear cell,PBMC),并做培养;检测PBMCs中IL-23R mRNA表达水平和PBMCs上清中IL-23和IL-17A水平;比较各组IL-23R mRNA表达水平,分析IL-23R表达水平对IL-17A水平的影响。结果 IL-23R mRNA表达水平:ATB组低于LTBI组(Z=-2.528,P=0.011),ATB组高于HD组(Z=-3.849,P<0.001);IL-17A水平:ATB组低于LTBI组(t=2.238,P=0.031),ATB组高于HD组(t=4.733,P<0.001);IL-23水平在三组中的差异无统计学意义(F=0.432,P=0.651);在ATB患者中,IL-23R mRNA表达水平与IL-17A水平呈正相关(r_s=0.438,P=0.047)。结论结核分支杆菌感染中,IL-23R的表达水平对Th17细胞介导的免疫应答起到调节作用,可能影响到TB的易感性和感染转归。Objective To investigate the effect of interleukin 23 receptor （IL-23R） on T helper cell 17 （Th17） call-mediated immune response in mycobacterium tuberculosis （TB） infection,and to explore the role of IL-23R in the pathogenesis of pulmonary tuberculosis.Methods 21 active lung tuberculosis （ATB） patients were enrolled in Beijing chest hospital from July to October in 2015,21 cases of latent tuberculosis infection （LTBI） and 21 healthy Healthy Donors （HD） were selected from Beijing Changping center for tuberculosis control and prevention from May to July in 2015.The peripheral blood mononuclear cells （PBMCs） were isolated and cultured.The expression of IL-23R mRNA in PBMCs was detected,IL-23 and IL-17A levels in the supernatant of PBMCs were measured.The expression of IL-23R mRNA in different groups and the effect of IL-23R expression on IL-17A level were analyzed.Results The expression of IL-23R mRNA in ATB group was lower than that in LTBI group （Z =-2.528,P =0.011）,and in ATB group was higher than that in HD group （Z =-3.849,P 〈 0.001）.The expression of IL-17A in ATB group was lower than that in LTB group （t =2.238,P =0.031）,and ATB group was higher than that in HD group （t =4.733,P 〈 0.001）.There was no significant difference in IL-23 level between the three groups （F =0.432,P =0.651）.IL-23R mRNA expression was positively correlated with IL-17A level （rs =0.438,P =0.047）.Conclusions The expression level of IL-23R in mycobacterium tuberculosis infection can regulate the immune response mediated by Th17 cells,which may affect the susceptibility and infection outcome of pulmonary tuberculosis.

关 键 词：结核 肺 RNA 信使 HLA-D抗原 分枝杆菌 结核 

分 类 号：R521[医药卫生—内科学] R378.911[医药卫生—临床医学]