空肠弯曲菌血红素氧化酶ChuZ促进巨噬细胞NLRP3炎性小体激活  

Campylobacter jejuni heme oxygenase ChuZ promotes inflammasome activation

作　　者：张睿[1] 熊怡淞[1] 刘媛[1] 江忠勇[1] 常凯[1] 胡宗海[1] 熊杰[1] ZHANG Rui XIONG Yisong LIU Yuan JIANG Zhongyong CHANG Kai HU Zonghai XIONG Jie(Clinical Laboratory, ChengDu Military General Hospital, Chengdu 610083, China)

机构地区：[1]中国人民解放军成都军区总院检验科,成都610083

出　　处：《免疫学杂志》2017年第11期955-959,共5页Immunological Journal

基　　金：国家自然科学基金(31400128)

摘　　要：目的探究空肠弯曲菌血红素氧化酶ChuZ对巨噬细胞炎性小体激活的影响。方法分离野生型C57BL/6小鼠骨髓细胞,诱导分化为骨髓来源的巨噬细胞(bone marrow-derived macrophage,BMM),分别用WT C.jejuni、△ChuZ C.jejuni以及对照刺激BMM 6 h,q RT-PCR和ELISA检测各组IL-1β的表达,q RT-PCR和Western blot检测各组NLRP3、ASC、caspase-1的表达。分离野生型C57BL/6和NLRP3-/-小鼠骨髓细胞,诱导分化为WT BMM、NLRP3-/-BMM,分别用WT C.jejuni和△ChuZ C.jejuni刺激BMM 6 h,ELISA检测各组IL-1β的表达。分离野生型C57BL/6小鼠骨髓细胞,诱导分化为BMM,分别用WT C.jejuni、△ChuZ C.jejuni刺激BMM、BMM+MCC950(NLRP3炎性小体抑制剂)6 h,ELISA检测各组IL-1β的表达。结果与对照组相比,WT C.jejuni、△ChuZ C.jejuni均能显著促进BMM IL-1β、NLRP3、procaspase-1、caspase-1的表达(P<0.01);△ChuZ C.jejuni组与WT C.jejuni组相比,IL-1β、NLRP3、procaspase-1、caspase-1的表达显著降低(P<0.01)。与WT BMM组相比,WT C.jejuni、△ChuZ C.jejuni分别刺激NLRP3-/-BMM后,IL-1β表达均显著降低(P<0.01)。同时,与control组相比,WT C.jejuni、△ChuZ C.jejuni刺激+MCC950组后,IL-1β表达均也显著降低(P<0.01)。结论空肠弯曲菌血红素氧化酶ChuZ与巨噬细胞中NLRP3/ASC/caspase-1炎性小体的激活程度相关,并促进巨噬细胞中IL-1β的表达,参与了空肠弯曲菌对宿主的炎性作用。This study aimed to explore the mechanism that Campylobacter jejuni heme oxygenase ChuZpromotes NLRP3 inflammasome activation. Mouse bone marrow was isolated and differentiated to bonemarrow-derived macrophage（BMM）, and then stimulated with WT C. jejuni,△ ChuZ C. jejuni or media. Theexpression of IL-1β, NLRP3, ASC, procaspase-1 and caspase-1 were detected by q RT-PCR, ELISA and Westernblotting, respectively. Compared with control group, WT C. jejuni, △ ChuZ C. jejuni significantly enhenced theexpression levels of IL-1β, NLRP3, procaspase-1 and caspase-1 in BMM cells; compared with WT BMM group, theprotein level of IL-1β significantly decreased in NLRP3-/-BMM cells stimulated with WT C. jejuni or △ ChuZ C.jejuni. Meanwhile, MCC950（NLRP3 inhibitor） inhibited IL-1β secretion level in BMM cells stimulated with WT C.jejuni, or △ ChuZ C. jejuni. Taken together, Campylobacter jejuni heme oxygenase ChuZ can promote IL-1βexpression, and is related to NLRP3/ASC/caspase-1 inflammasome activation levels, thus may take part in thedevelopment and progression of Campylobacter jejuni-induced inflammation.

关 键 词：空肠弯曲菌 ChuZ 炎性小体 IL-1Β NLRP3 

分 类 号：R392[医药卫生—免疫学]