β-榄香烯对RNA聚合酶活性的抑制及与DNA的结合  

THE EFFECTS OF β-ELEMENE ON DNA-BINDING SPECIFICITY AND RNA POLYMERASE INHIBITORY ACTIVITY

作　　者：崔秀云[1] 李德山 

机构地区：[1]大连医学院生化教研室,116023 [2]大连市医药科学研究所,116013

出　　处：《中国药理学通报》1991年第3期228-231,共4页Chinese Pharmacological Bulletin

摘　　要：β-榄香烯是中药温莪术中的一种有效成分,它能抑制某些肿瘤的增长。我们的实验表明,β-榄香烯浓度为0.0074mol/L至0.0172mol/L时对RNA聚合酶有明显的抑制作用;加入β-榄香烯后DNA融点下降,吸收光谱移位及荧光强度增加,表明本品与DNA相结合。β-elemene that is an element of the Essential oil of the Cuicu-ma Wenyujin showed appreciable anticancer ability. The experimental results showed that the RNA polymerase activity was inhibited by β-elemene at concentrations of 0.0074 mol/L^0.0172 mol/L. The study of thermal denaturation indicated that the Tm of DNA was decreased after adding β-elemene. The fluorescent analysis of β-elemene bound to DNA showed that the intensity of fluorescence was increased. The peak of absorption of DNA was shifted from 260 nm to 240 nm after treatment with β-elemene.

关 键 词：Β榄香烯 RNA聚合酶 DNA融点 

分 类 号：R963[医药卫生—微生物与生化药学]