参茸益精片对急性高原缺氧脑损伤的保护作用研究  

Protective effect of Shen Rong Yi Jing tablets on acute high-altitude hypoxia brain injury

作　　者：王海伟 王自勤 苏丹 霍坚 吕宏迪 WANG Hai-wei WANG Zi-qin SU Dan HUO Jian LV Hong-di(159 Hospital of PLA,Zhumadian 463000, China Armed Police Corps Hospital of Henan ,Zhengzhou 450052, China)

机构地区：[1]解放军第一五九医院,河南驻马店463000 [2]河南省武警总队医院,河南郑州450052

出　　处：《实用药物与临床》2017年第10期1112-1115,共4页Practical Pharmacy and Clinical Remedies

基　　金：河南省科技立项课题(162102310264);军队后勤科技立项课题(CJN15J090)

摘　　要：目的观察参茸益精片对急性高原缺氧脑损伤的保护作用,并探讨其分子机制。方法将SD大鼠随机分为空白对照组(对照组)、高原急性缺氧模型组(模型组)和参茸益精片治疗组(治疗组),每组6只。建立急性高原缺氧脑损伤大鼠动物模型,治疗组灌胃参茸益精片。观察大鼠脑组织SOD活性和MDA水平的变化,Western blot和RT-PCR检测胆碱乙酰转移酶(Choline acetyltransferase,ChAT)和蛋白激酶C(Protein kinase C,PKC)表达水平的变化。结果与对照组比较,模型组大鼠脑组织SOD活性显著降低(P<0.01),MDA水平显著增加(P<0.01);治疗组大鼠脑组织SOD活性显著增加(P<0.01),MDA水平显著降低(P<0.01)。模型组大鼠脑组织ChAT和PKC表达水平显著降低(P<0.05),治疗组ChAT和PKC表达水平显著升高(P<0.05)。结论参茸益精片可以保护急性高原缺氧引起的脑损伤,可能与其调节ChAT和PKC的表达水平有关。Objective To observe the protective effect of Shen Rong Yi Jing tablets on acute high-altitude hypoxia brain injury, and explore its molecular mechanism. Methods SD rats were randomly divided into blank control group（ control group）,acute altitude hypoxia model group（ model group） and Shen Rong Yi Jing tablets treatment group（ treatment group）,6 rats in each group. The rat model of acute high-altitude hypoxia brain injury was set up and the SOD and MDA level was detected after treatment with Shen Rong Yi Jing tablets. The expression of ChAT and PKC was evaluated by Western blot and RT-PCR. Results Compared with control group, the activity of SOD in the brain tissue of model group decreased significantly（ P〈0. 01）,while the level of MDA increased significantly（ P〈0. 01）; the activity of SOD in the brain tissue of rats in treatment group increased significantly（ P〈0. 01）,while the level of MDA decreased significantly（ P〈0. 01）. The expression of ChAT and PKC in brain tissue of model group decreased significantly（ P〈0. 05）,while the expression level of ChAT and PKC in treatment group increased significantly（ P〈0. 05）. Conclusion Shen Rong Yi Jing tablets can protect brain damage caused by acute high-altitude hypoxia,which may be related to the regulation of the expression levels of ChAT and PKC.

关 键 词：参茸益精片 急性高原缺氧 脑损伤 胆碱乙酰转移酶 蛋白激酶C 

分 类 号：R285.5[医药卫生—中药学]