地黄叶对糖尿病肾病大鼠肠道菌群的调节作用  

Regulatory effect of the leaves of Rehmannia glutinosa Libosch on intestinal microflora in diabetic nephropathy rats

作　　者：戴新新 蔡红蝶 宿树兰[1] 郑天瑶 魏丹丹[1] 严辉[1] 朱悦[1] 钱大玮[1] 段金廒[1] 

机构地区：[1]南京中医药大学,江苏省中药资源产业化过程协同创新中心,中药资源产业化与方剂创新药物国家地方联合工程研究中心,国家中医药管理局中药资源循环利用重点研究室,江苏南京210023

出　　处：《药学学报》2017年第11期1683-1691,共9页Acta Pharmaceutica Sinica

基　　金：江苏省方剂高技术研究重点实验室建设项目(BM2010578);江苏省中药学优势学科Ⅱ期建设项目(ysxk-2014);江苏省中药资源产业化过程协同创新中心重点项目(ZDXM-3-7);江苏省“333高层次人才培养工程”资助项目(BRA2015391)

摘　　要：研究地黄叶总苷提取物及地黄叶总苷胶囊对糖尿病肾病大鼠肠道菌群的调节作用。将48只大鼠随机分为正常组(C)、模型组(M)、黄葵胶囊组(0.75 g·kg^(-1)·d^(-1),HK)、厄贝沙坦组(27 mg·kg^(-1)·d^(-1),YX)、地黄叶总苷提取物低剂量组(4.3 g·kg^(-1)·d^(-1),DHYL)、地黄叶总苷提取物高剂量组(7.2 g·kg^(-1)·d^(-1),DHYH)、地黄叶总苷胶囊低剂量组(216 mg·kg^(-1)·d^(-1),JNL)和地黄叶总苷胶囊高剂量组(360 mg·kg^(-1)·d^(-1),JNH),每组6只。除正常组外,其余各组采用高糖高脂饲料+5%葡萄糖饮水+注射链脲佐菌素(45 mg·kg^(-1),STZ)的方法复制糖尿病肾病模型。造模成功后,各组药物给药2周,通过16S rDNA测序的方法研究地黄叶总苷提取物和地黄叶总苷胶囊对糖尿病肾病大鼠肠道菌群的调节作用。结果表明,与正常组相比,糖尿病肾病模型组大鼠肠道内菌群从门水平到属水平均发生了显著变化;且模型组大鼠肠道菌中乳酸杆菌属比例显著降低;给药后乳酸杆菌属比例有所回升,尤其是厄贝沙坦组、地黄叶总苷提取物低剂量组和地黄叶总苷胶囊低剂量组调节作用较为显著。可见,地黄叶总苷具有改善STZ诱导的糖尿病肾病大鼠肠道菌群失调的作用。The aim of this study was to investigate the regulatory effect of the total glycoside extracted from leaves of Rehmannia（TLR）and Dihuangye total glycoside capsule（DTG）on intestinal microflora in diabetic nephropathy rats.Forty-eight rats were randomly divided into the control group（C）,model group（M）,Huangkui capsule group（0.75 g·kg^（-1）·d^（-1）,HK）,irbesartan group（27 mg·kg^（-1）·d^（-1）,YX）,TLR low dose group（4.3 g·kg^（-1）·d^（-1）,DHYL）,TLR high dose group（7.2 g·kg^（-1）·d^（-1）,DHYH）,DTG low dose group（216 mg·kg^（-1）·d^（-1）,JNL）,DTG high dose group（360 mg·kg^（-1）·d^（-1）,JNH）.Rat model of diabetic nephropathy was induced by intraperitoneal injection of small dose of streptozotocin（45 mg·kg^（-1）,STZ）and feeding high-fat diet and 5% glucose drinking water.After oral administration for two weeks,the 16S rDNA sequencing method was used to study the effects of the TLR and DTG on intestinal flora in diabetic nephropathy rats.The results showed that compared with the control group,the intestinal flora of diabetic nephropathy rats had changed from phylum units to the genus units.Moreover,the proportion of lactobacilli in the intestinal bacteria of the model group was significantly decreased,and the proportion of lactobacilli in the administration group was increased,especially the YX group,TLR low dose group and DTG low dose group.The data suggest that the total glycosides of Rehmannia glutinosa improved the disorder of intestinal flora in STZ-induced diabetic nephropathy rats.

关 键 词：地黄叶 总苷 糖尿病肾病 肠道菌群 

分 类 号：R285[医药卫生—中药学]