2型糖尿病患者外周血单核细胞亚群脂蛋白相关磷脂酶A2基因表达研究  

Expression of LP-PLA2 mRNA in peripheral blood monocytes subsets of patients with type 2 diabetes mellitus

作　　者：孙明忠[1] 周中卫[1] 季禹乔[1] 朱晖[1] 江冬梅[2] 陈红梅[1] 

机构地区：[1]东南大学医学院附属盐城医院检验科,224001 [2]东南大学医学院附属盐城医院内分泌科,224001

出　　处：《中华内分泌代谢杂志》2017年第10期822-827,共6页Chinese Journal of Endocrinology and Metabolism

摘　　要：目的通过检测2型糖尿病患者外周血单核细胞亚群脂蛋白磷脂酶A2（LP-PLA2）mRNA表达，探讨单核细胞亚群在2型糖尿病发病中的作用。方法收集32例2型糖尿病患者和22名健康对照者外周血，流式细胞仪检测单核细胞亚群比例，PCR检测单核细胞亚群LP-PLA2 mRNA表达。健康人单核细胞亚群在体外诱导为巨噬细胞，经2型糖尿病患者血清刺激24h后检测LP-PLA2 mRNA表达。结果与对照组相比，2型糖尿病患者经典型单核细胞比例降低，非经典型、中间型单核细胞比例及其LP-PLA2 mRNA水平显著升高（P〈0.05）；2型糖尿病患者稳态模型评估的胰岛素抵抗指数与非经典型（P〈0.05）和中间型单核细胞（P〈0.01）LP-PLA2 mRNA水平呈正相关；伴颈动脉粥样硬化2型糖尿病患者与单纯糖尿病患者相比，中间型和非经典型单核细胞LP-PLA2 mRNA水平明显升高（P〈0.05）。巨噬细胞经2型糖尿病患者血清刺激后，非经典型单核细胞LP-PLA2 mRNA水平高于对照组（P〈0.05）。结论2型糖尿病患者外周血单核细胞向中间型和非经典型极化，且高表达LP-PLA2 mRNA，可能参与2型糖尿病和动脉粥样硬化的发生。Objective To investigate the role of monocyte subsets in the pathogenesis of type 2 diabetes mellitus（T2DM）, the expression was detected on lipoprotein-associated phospholipase A2（LP-PLA2）mRNA in peripheral blood monocytes subsets of type 2 diabetic patients. Methods After the peripheral blood of 32 type 2 diabetic patients and 22 healthy controls were collected, the percentages of the monocytes subsets were detected by flow cytometry. The expression of LP-PLA2 mRNA in monocytes subsets were detected by PCR. After monocyte subsets of healthy subjects were induced into macrophages and stimulated by the serum of type 2 diabetic patients for 24h, LP-PLA2 mRNA expression was detected.Results Compared with the control group, the percentage of classical monocytes in T2DM group was decreased, while the LP-PLA2 mRNA expression and percentage of nonclassical, intermediate monocytes were significantly increased（all P〈0.05）. In addition, the homeostasis model assessment for insulin resistance index was positively correlated with LP-PLA2 mRNA expression in non-classical（P〈0.05）and intermediate monocytes（P〈0.01）of type 2 diabetic patients. Compared with type 2 diabetic patients without carotid atherosclerosis, the levels of LP-PLA2 mRNA in intermediate and nonclassical monocytes were significantly higher than those in type 2 diabetic patients with carotid atherosclerosis（P〈0.05）. The level of LP-PLA2 mRNA in non-classical monocytes-derived macrophages stimulated with the serum of type 2 diabetic patients was significantly higher than that in the control group（P〈0.05）.Conclusion Monocytes subsets from type 2 diabetic patients are abnormally skewed toward to intermediate and non-classical monocytes with high expression of LP-PLA2 mRNA, which may play an important role in the pathogenesis of type 2 diabetes mellitus and atherosclerosis.

关 键 词：糖尿病 2型 单核细胞亚群 脂蛋白相关磷脂酶A2 动脉粥样硬化 

分 类 号：R446.1[医药卫生—诊断学] R587.1[医药卫生—临床医学]