鼠妇抗肿瘤活性物质初步探究  

Preliminary Extraction of Anti-tumor Substances Derived from Porcellio Scaber Latreille

作　　者：刘文静[1] 杨稳[1] 刘飞[1] 周长征[1] 

机构地区：[1]山东中医药大学,山东济南250000

出　　处：《辽宁中医药大学学报》2017年第10期15-18,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：山东省中医药抗病毒应用基础与关键技术研究及产业化课题(XTCΧ2014C01-04)

摘　　要：目的:通过体外抗肿瘤实验选择鼠妇抗肿瘤活性物质的提取方法和敏感细胞株,为筛选抗肿瘤活性物质做准备。方法:采用水回流提取、水浸渍提取、95%乙醇回流提取、乙醇浸渍提取、仿生酶解提取的方法对鼠妇粉末进行处理,并将其提取物对人肺腺癌细胞(A549)、肝癌细胞(BEL-7402)、乳腺癌细胞(MCF-7)和喉癌细胞(Hep-2)5种癌细胞进行体外抗肿瘤实验,同时采用MTT细胞增殖检测的方法对其抗肿瘤效果进行追踪,确定最佳提取方法和敏感细胞株后,对该方法的提取物用氯仿、正丁醇进一步萃取,再将各萃取部位用敏感细胞株分别进行体外抗肿瘤实验,筛选出最佳抗肿瘤活性部位。结果:与其余4种方法的提取物相比,乙醇回流提取物对A549、BEL-7402、MCF-7、Hep-2 4种癌细胞的抑制作用最好,IC50分别为185.99、119.80、52.94、193.08μg/m L,该提取物的氯仿萃取物对MCF-7的抑制作用明显高于正丁醇萃取物,IC50分别为58.13、190.47μg/m L。结论:乙醇回流提取物对多种癌细胞均有抑制作用,其抗肿瘤活性物质集中在氯仿萃取物中。Objective ：To prepare for the screening of anti-tumor active substances,in vitro antitumor experiments were used to screen the anti-tumor active substances of Porcellio scaber Latreille and sensitive cell lines. Methods ：In this study,the Latreille were extracted by five methods（water reflux,water immersion,95% ethanol reflux,ethanol dipping and biomimetic enzymatic）,and the extracts were tested on human lung adenocarcinoma cells（A549）,hepatocellular carcinoma（BEL-7402）,breast cancer cells（MCF-7）and laryngeal carcinoma cells（Hep-2）,and the anti-tumor effect was tracked by MTT cell proliferation assay. After the best extraction method and sensitive cell lines were determined,the extract was extracted with chloroform and n-butanol,and then the in vitro anti-tumor experiments were carried out with the sensitive cell lines,and the best active sites were screened out. Results ：Compared with the other four methods,the ethanol extract had the best inhibitory effect on A549,BEL-7402,MCF-7 and Hep-2 cells,the IC50 was 185.99,119.80,52.94,193.08 μg/m L,The inhibitory effect of the extract on MCF-7 was significantly higher than that of n-butanol extract,IC50 was 58.13μg/m L and 190.47μg/m L,respectively. Conclusion ：Ethanol reflux extract have inhibition to many kinds of cancer cells,and its anti-tumor active substance is concentrated in chloroform extract.

关 键 词：鼠妇 抗肿瘤活性 提取物 

分 类 号：R285.5[医药卫生—中药学]