姜黄素-槲皮素复方自微乳制备与评价研究  

Preparation and Quality Evaluation of Curcumin and Quercetin Loaded Self-microemulsifying Drug Delivery System

作　　者：黄瑞雪[1] 茅玉炜 黎翊君 刘乐环 余家齐 王渐鸿 戴俊东[1] 

机构地区：[1]北京中医药大学,北京100102

出　　处：《辽宁中医药大学学报》2017年第10期33-36,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：北京中医药大学自主选题项目(2016-JYB-XS072)

摘　　要：目的:制备姜黄素-槲皮素自微乳(CUR-QUE-SMEDDS)给药体系并建立其质量评价方法。方法:选择Capryol 90为油相,Cremophor RH40为表面活性剂,Transcutol HP为助表面活性剂,通过单因素实验优化处方,制备CUR-QUE-SMEDDS。以自微乳的外观性状、粒径分布、Zeta电位、微观形态、包封率和载药量为指标对其进行质量评价。结果:制备的CUR-QUE-SMEDDS为澄明液体,流动性、稳定性良好,遇水可自发形成O/W型微乳,平均粒径(6.90±0.09)nm;Zeta电位(-7.13±2.25)m V。HPLC法检测姜黄素、槲皮素的线性范围分别为10.728~96.552μg·mL^(-1)和1.064~9.576μg·mL^(-1),r=0.9998和r=0.9999。姜黄素和槲皮素的平均回收率分别为99.98%和100.34%;RSD分别为1.46%和1.06%。CUR-QUE-SMEDDS中姜黄素、槲皮素的包封率和载药量分别为95.97%±0.50%、(25.82±0.15)mg·g^(-1),95.51%±2.52%、(1.80±0.05)mg·g^(-1)。结论:CUR-QUE-SMEDDS制备工艺简单、包封率高,粒径分布均匀。Objective ：The objective of the study was to prepare and evaluate the quality of curcumin and quercetin loaded self-microemulsifying drug delivery system（CUR-QUE-SMEDDS）. Methods ：Based on the curcumin self-microemulsifying drug delivery system（CUR-SMEDDS）,the CUR-QUE-SMEDDS composed of Capryol 90（10.0%）,Cremophor RH40（60.0%）,Transcutol HP（30.0%）. The optimal prescription（CUR-QUE-SMEDDS）was confirmed by single-factor design experiment,using appearance status,particle diameter,Zeta potential,transmission electron microscope micrographs,drug entrapment efficiency and the drug loadings of curcumin and quercetin as evaluation indexes to determine the quality evaluation of Cur-PIP-SMEDDS. Results ：The CUR-QUE-SMEDDS prepared was a translucent and achromatous solution with good stability. After being diluted with water,CUR-QUE-SMEDDS was consisted of small spherical drops. The average particle size was（6.90±0.09）nm,Zeta potential was（-7.13±2.25）m V. The calibration curves were linear in the range of 10.728~96.552 μg·mL^（-1）（r=0.9998）for CUR and 1.064~9.576 μg·mL^（-1）（r=0.9999）for QUE. The average recovery was in the range of 99.98% and 100.34%,respectively. The EE and DL of the final prescription,CUR was（95.97%±0.50%）（25.82±0.15）mg·g^（-1）,QUR was（95.51%±2.52%）（1.80±0.05）mg·g^（-1）. Conclusion ：The preparation technology of CUR-QUE-SMEDDS is simple and feasible. The entrapment efficiency of CURQUE-SMEDDS is good and its particle size distribution is uniform.

关 键 词：姜黄素 槲皮素 复方自微乳 质量评价 

分 类 号：R284[医药卫生—中药学]