不同产地远志中远志酮Ⅲ、3,6'-二芥子酰基蔗糖、细叶远志皂苷A含量测定  

Determination of Polygalaxanthone Ⅲ,3,6'-disinapoyl Sucrose,and Tenuifoliside A in Polygalae Radix from Different Growing Areas by HPLC

作　　者：王海波[1] 刘勇[2] 矫承媛[3] 邸学[1] 王媛媛 于新竹 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]大连医科大学附属第一医院,辽宁大连116011 [3]大连市儿童医院,辽宁大连116012

出　　处：《辽宁中医药大学学报》2017年第10期39-41,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：辽宁中医药大学中医脏象理论及应用国家教育部重点实验室开放基金项目(zyzx1610);沈阳市科技创新智库项目(sz20160731)

摘　　要：目的:建立同时测定山西、甘肃等不同省区产远志中远志酮Ⅲ、3,6'-二芥子酰基蔗糖、细叶远志皂苷A 3种成分含量的HPLC测定方法。方法:超声提取,ODS-C18色谱柱,柱温35℃,流动相为0.05%磷酸乙腈溶液(A)-0.05%磷酸水溶液(B),梯度洗脱;检测波长315 nm。结果:远志中3种成分在30 min内良好分离,其它成分在65 min内基本分离。3种成分在0.002~1.0μg质量范围内线性关系良好、平均加样回收率95.3%~109.0%。结论:不同产地中远志酮Ⅲ、3,6'-二芥子酰基蔗糖、细叶远志皂苷A含量范围分别为1.01~7.47、2.44~5.63、2.13~7.19 mg·g^(-1)。方法操作简便,结果准确,可用于远志药材3种成分含量测定。Objective ：To develop a quantitative HPLC method for quantitative analysis of three components in Polygala tenuifolia collected from different growing areas. Methods ：The P. tenuifolia extract was separated by ODS-C18. The mobile phase of acetonitrile with 0.05% phosphoric acid（A）and 0.05% phosphoric acid water（B）with gradient elution,detection wavelength was 315 nm. Results ：In the ranges of 0.002-0.1 μg,3,6＇-disinapoyl sucrose（DSS）,polygalaxanthone Ⅲ（PXⅢ）,and tenuifoliside A（TFA）were in linear relationships. Their mean recoveries were at 95.3%-109.2%. Conclusion ：The contents of DSS,PXⅢ,and TFA in P. tenuifolia pieces were in the ranges of 1.01-7.47,2.44-5.63,2.13-7.19 mg·g-1respectively. The method is simple to operate and accurate,it can be used to determine thd content of 3 componets in P. tenuifolia.

关 键 词：远志 含量 远志酮Ⅲ 3 6'-二芥子酰基蔗糖 细叶远志皂苷A 

分 类 号：R284[医药卫生—中药学]