肉兔Follistatin基因的表达研究  

Study on Follistatin gene expression in meat rabbit

作　　者：郑洁[1,2] 谢晓红 雷岷[1,2] 张翔宇 邝良德[1,2] 杨超 任永军[1,2] 

机构地区：[1]四川省畜牧科学研究院,成都610066 [2]动物遗传育种四川省重点实验室,成都610066

出　　处：《黑龙江畜牧兽医》2017年第11期221-224,299,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家兔产业技术体系项目(CARS-44-B-4);四川省公益性科研院所基本科研业务费项目(SASA2014A12);肉兔配套系培育及配套技术研究与示范项目(SASA2014CZYX005);优质肉兔配套系选育及配套技术研究与示范项目(2011NZ0099);家兔现代产业链关键技术集成研究与产业化示范项目(2016NZ0002-01)

摘　　要：为了揭示肉兔卵泡抑素(Follistatin)基因对骨骼肌发育的作用,试验采用实时荧光定量PCR(qRT-PCR)方法,以加利福尼亚兔(U兔)和齐卡巨型白兔(G兔)为研究对象,检测了不同品种、不同性别、不同日龄肉兔Follistatin基因在股二头肌中的表达量,测定个体活体重和日增重。结果表明:300日龄时G兔Follistatin基因表达量极显著高于U兔(P<0.01),不同日龄公兔Follistatin基因表达量均高于母兔,35和56日龄公兔股二头肌中Follistatin基因表达量极显著高于母兔(P<0.01),并且Follistatin基因在两个品种肉兔股二头肌中的表达变化趋势较为一致,即随个体日龄增长呈现M型趋势。股二头肌中Follistatin基因发育变化与活体重呈负相关,与日增重的变化呈显著正相关(P<0.05)。说明Follistatin基因在肉兔的骨骼肌发育过程中具有重要的调控作用。In order to explore the function of Follistatin gene in the development of skeletal muscle ,real - time PCR method was used in this ex- periment, and Californian rabbit（ U rabbit）and Zika giant rabbit （G rabbit）were research objects. Expression contents of Follistatin gene were detected from biceps femoris of meat rabbit with different breeds, different sex and different days old ; and individual live weight and daily gain were measured. The results showed that the expression of Follistatin gene in 300 day - old G rabbits was significantly higher than that in U rab- bits（ P 〈 0.01 ）. The expression of Follistatin gene in male was higher than that in female with different days old ; on 35 or 56 days old the ex- pression of Follistatin gene from biceps femoris in male was significantly higher than that in female （P 〈 0.01 ）. Expression trend of Follistatin gene from biceps femoris had no difference between the breeds ,which was a M -shape trend with individual day -old growth. Negative correla- tion existed between Follistatin gene expression from biceps femoris and live weight;positive correlation appeared between the gene expression and daily gain（ P 〈 0.05 ）. The results suggested that Follistatin gene had an important role in skeletal muscle development of meat rabbits.

关 键 词：卵泡抑素(Follistatin) 股二头肌 实时荧光定量PCR(qRT—PCR) 加利福尼亚兔 齐卡巨型 白兔 

分 类 号：S829.1[农业科学—畜牧学] Q78[农业科学—畜牧兽医]