山羊源第三梭菌的分离鉴定及耐药性分析  

Isolation and identification of Clostridium tertium from goat and its drug resistance analysis

作　　者：董文龙[1] 王巍[1] 耿昕颖 王羽[1] 张红伟[1] 孙健[2] 马红霞[1] 高云航[1] 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118 [2]吉林省畜牧兽医科学研究院,吉林长春130062

出　　处：《中国兽医学报》2017年第11期2137-2140,共4页Chinese Journal of Veterinary Science

基　　金：吉林省现代农业产业技术体系建设专项(2015-24);国家科技支撑计划项目(2014BAK19B005)

摘　　要：为确定导致山羊严重腹泻的病原菌,从病死山羊肠道内分离到的1株优势菌并命名为CT-1,根据其形态特征、培养特性、生化特性及16SrDNA分子鉴定结果确定分离菌株为第三梭菌。随后对分离菌株进行了动物回归致病性试验及药敏试验。动物回归致病性试验表明,该分离株能够导致山羊严重腹泻。药敏结果显示,该菌对氯霉素,红霉素,庆大霉素,阿米卡星敏感;对恩诺沙星,环丙沙星,阿奇霉素,强力霉素,氟苯尼考,阿莫西林,磺胺间甲氧嘧啶耐药。In order to identify the pathogen of the goat with a bad attack of diarrhoea. A dominant bacterium, named as CT-1, was isolated from intestinal tract of dead goats. The isolated strain identified as Clostridium tertiurn by morphological characteristics, biochemical test and bacteria 16S rDNA molecular biological experiments, whereafter,animal pathogenicity test and drug sensi- tive test were carried out. Animal pathogenicity test showed that the isolated strain had a strong pathogenicity to goat. Drug sensitive test results showed that the isolated strain was sensitived to chloramphenicol, erythromycin,gentamicin and amikacin; but was resistanted to enrofloxacin, ciprofloxaein, azithromycin, doxycycline, florfenicol, amoxicillin and sulfamonomethoxine.

关 键 词：山羊 第三梭菌 分离鉴定 耐药性分析 

分 类 号：S852.61[农业科学—基础兽医学]