代综方对C2C12骨骼肌细胞葡萄糖摄取及GLUT4蛋白表达的影响  

Effects of DZF on glucose uptake and GLUT4 protein expression in C2C12 myotubes

作　　者：赵玉雪[1] 朱晓云[1] 袁泉 刘喜明[1] 

机构地区：[1]中国中医科学院广安门医院内分泌科,北京100053

出　　处：《重庆医科大学学报》2017年第11期1494-1497,共4页Journal of Chongqing Medical University

基　　金："十二五"重大新药创制资助项目(编号:2011ZX09102-011-08);中英国际合作资助项目(编号:2010DFA31620)

摘　　要：目的:研究代综方(dai zong fang,DZF)对骨骼肌细胞葡萄糖摄取及葡萄糖转运体4(glucose transporter 4,GLUT4)蛋白表达的影响。方法:采用含2%马血清(horse serum,HS)的DMEM培养液诱导C2C12骨骼肌细胞分化为肌管,荧光标记的葡萄糖类似物2-NBDG测定葡萄糖摄取,对2-NBDG的量效时效关系进行测定;采用不同剂量DZF干预分化的C2C12细胞,检测其对细胞基础状态及胰岛素刺激状态下葡萄糖摄取的影响;Western blot检测DZF对C2C12细胞GLUT4总蛋白表达及膜转位的影响。结果:在基础状态或胰岛素刺激下,DZF低、中、高剂量均可提高骨骼肌葡萄糖摄取,差异有统计学意义(P<0.05)。基础状态下,DZF能促进GLUT4总蛋白及膜转位蛋白的表达(P<0.05);胰岛素刺激状态下,DZF对GLUT4总蛋白表达无显著影响(P>0.05),但仍可促进GLUT4膜转位蛋白的表达(P<0.05)。结论:DZF可增加骨骼肌细胞葡萄糖摄取,具有类胰岛素作用,其作用机制可能与促进GLUT4蛋白向细胞膜转位有关。Objectives：To investigate the effects of dai zong fang（DZF） on glucose uptake and glucose transporter 4 protein expression in skeletal muscle cells. Methods：C2C12 skeletal muscle cells were differentiated into myotubes by incubating in DMEM with 2% horse serum;glucose uptake in C2C12 myotubes were measured by 2-NBDG;the dose effect and time effect of 2-NBDG were ana- lyzed. After the intervention of different concentrations of DZF, glucose uptake in C2C12 myotubes were analyzed in basal and in- sulin-stimulated state. Effects of DZF on GLUT4 protein expression and translocation were analyzed by Western blot. Results： Both in basal and insulin-stimulated state,different concentrations of DZF could stimulate the glucose uptake in skeletal muscle cells,with statistically significant difference（P〈0.05）. In the basal state, DZF significantly increased total and membrane GLUT4 protein expres- sion （P〈0.05）. In the insulin-stimulated state, DZF had no significant effect on the total GLUT4 protein expression （P〉0.05）, however, it could significantly promote the membrane GLUT4 protein expression（P〈O.05）. Conclusion：DZF could increase glucose uptake in skeletal muscle cells and have insulin-like function,the mechanisms might be related to the promotion of GLUT4 trauslocation.

关 键 词：代综方 2-NBDG C2C12细胞 葡萄糖转运体4 

分 类 号：R587.1[医药卫生—内分泌]