四氯化碳气体对初代培养大鼠肝细胞的损害  

THE TOXICITY OF VOLATILIZED CARBON TETRACHLORIDE IN PRIMARY CULTURES OF RAT HEPATOCYTES

作　　者：张罗修[1] 潘德济 曹致芳[1] 

机构地区：[1]上海医科大学药学院药理教研室,200032 [2]上海医科大学药学院天然药化教研室,200032

出　　处：《中国药理学通报》1991年第3期187-189,共3页Chinese Pharmacological Bulletin

基　　金：中国科学院基金资助课题

摘　　要：研究四氯化碳(CCl_4)挥发气体在试管内对大鼠初代培养肝细胞的损害,导致谷丙转氨酶(GPT)外逸;观察了影响CCl_4作用的因素,并初步观察了几种药物在试管内对CCl_4毒性的影响,发现咖啡酸钠盐在10mg/L以上,甘草甜素铵盐在100mg/L可使GPT明显降低,韭叶柴胡多糖在类似浓度则无明显作用。上述结果表明离体的初代培养大鼠肝细胞为研究肝毒剂的毒理作用,护肝药物的作用原理及肝细胞的代谢提供了良好的研究系统。The leakage of glutamic-pyruvic transaminase ( GPT ) in the supernatant of primary cultures of rat hepatocytes has been used to evaluate the toxicity of the classical hepatotoxin carbon tetrachloride ( CC14 ) . The response of the isolated hepatocyte's preparation to incubate with CCl4 showed a clear dose dependency from 5 to 20 ml/ L in this system and the toxicity increased -with time of incubation. Glycyrrhizin ( 100mg/L ) and sodium caffeic acid 10 to 100 mg/L remarkably reduced GPT release in the medium of hepatocytes with CC14 while Bupleurum kunmingense polysaccharides had no significant effect at the same condition. Glycyrrhizin and caffeic acid might have protective effects against CC14. Further study is necessary. These findings also indicated that the primary cultures of rat hepatocytes is a suitable system for evaluating liver protective drugs as well as studying the function of cell metabolism.

关 键 词：肝细胞 四氯化碳 谷丙转氨酶 

分 类 号：R965.1[医药卫生—药理学]