中医不同治法对NAFLD大鼠血脂及肝功能影响的实验研究  

Experimental study on the effect of Traditional Chinese Medicine Different-methods treating blood-fat and hepatic function in NAFLD rats

作　　者：黎运呈[1] 丁贤君[1] 郑迪 

机构地区：[1]舟山市人民医院,浙江舟山316004

出　　处：《甘肃医药》2017年第10期804-807,共4页Gansu Medical Journal

基　　金：浙江省中医药科技计划项目(项目编号:2013ZQ029)

摘　　要：目的:研究不同的中医治法对大鼠非酒精性脂肪性肝病(NAFLD)模型各时期甘油三酯及转氨酶等指标干预的机制,为临床中医药防治NAFLD提供理论依据。方法:取SPF级Wistar大鼠80只,随机分为5组,造模。造模第4周后,各组加用中医不同治法进行药物干预。在实验第8、12周时,处死大鼠,测定肝功能、炎症因子及肝指数等指标。结果:实验第8周时,治疗Z方组改善NAFLD大鼠肝功能及甘油三酯的疗效显著优于其他药物治疗组(P<0.05),降低血脂及炎症指标的疗效与其他药物治疗组进行统计学比较,有极显著差异(P<0.01),且能有效改善肝指数。在实验第12周时,治疗H方组改善大鼠NAFLD模型血清谷丙转氨酶、TNF-α的效果显著优于其他药物治疗组(P<0.05),降低血清FFA、肝指数及谷丙转氨酶的效果与其他药物干预治疗组进行统计学比较有极显著性差异(P<0.01),并且有明显降低血LDL、TG、TCH指标的效果。结论:在NAFLD的初期阶段,运用具有活血利湿、化痰消瘀、健脾疏肝功效的药物Z方对改善血脂、TNF-α、肝指数及肝功能等指标的疗效优于其他药物治疗;在NAFLD的后期,采用具有清利湿热、化痰消瘀、补益肝肾作用的中药H方对改善细胞炎症因子水平、肝指数及转氨酶等指标的疗效优于其他药物治疗。Objective：To explore the function mechanism of Traditional Chinese Medicine Different-method treating blood-fat and hepatic function in nonalcoholic fatty liver disease（NAFLD） rats’various times,which provide the basis theory to prevent and cure the NAFLD in clinic. Methods：Fetched 80 Wistar rats’ level of SPF,were divided into 5 groups at random. After 4 weeks later,Different Medicine were interfered each group. When the experiment were in 8,12 weeks,the above each group took 8 rats,we determined the blood serum value of ALT,AST,FFA,TG,TCH,LDL ,TNF-α and liver index in rat. Results：①At the end of the 8 weeks,the group number of 4 had better curative effect than other treatment groups at reducing the blood serum value of ALT,AST,TG,TCH（P〈0.05）,FFA,LDL ,TNF-α,TCH（P〈0.01） and Could reduce effectively liver index in rat.②At the end of the 12 weeks,the group number of 5 had better curative effect than other treatment groups at reducing the blood serum value of AST,TNF-α（P〈0.05）,ALT,FFA ,liver index（P〈0.01） and had tendency of reducing the blood serum value of LDL ,TG,TCH in rat. Conclusions：At early stage of NAFLD,Medicine A having effectiveness of dispersing the depressed liver-energy to invigorate the spleen,activating blood circulation to dissipate blood stasis and dissipating phlegm to decrease wet had better curative effect on blood-fat,Cell inflammatory factor,Liver index and Hepatic function;At later stage of NAFLD,Medicine B having effectiveness of dissipating phlegm to dissipate blood stasis,clearing away heat evil to decrease wet and benefiting hepatorenal function had better curative effect on Cell inflammatory factor,Liver index and Hepatic function than other medicines.

关 键 词：中医不同治法 肝指数 大鼠血脂 肝功能 非酒精性脂肪性肝病 

分 类 号：R259[医药卫生—中西医结合]