白介素(IL)-32在胆道闭锁患儿肝组织中的表达水平及临床意义  

Exresssion Level and Clinical Significance of Interleukin-32(IL-32) in Liver Tissues of Biliary Atresia Patients

作　　者：叶晓烁[1] 吴宙光[1] 王建尧[1] 

机构地区：[1]广东省深圳市儿童医院小儿外科,广东深圳518000

出　　处：《黑龙江医药》2016年第4期642-646,共5页Heilongjiang Medicine journal

摘　　要：目的：研究白介素-32（interleukin-32,IL-32）在胆道闭锁（biliary atresia,BA）及胆总管扩张（choledochal cyst,CC）患儿肝脏组织中的表达水平变化,从而探讨白介素（IL）-32在胆道闭锁患儿肝脏组织中的临床意义。方法：选取我院2012年2月~2015年10月期间收治的胆道闭锁患儿35例及未发生胆管炎的胆管扩张症患儿14例为研究对象,划分胆道闭锁患儿为观察组（BA）及胆管扩张患儿为对照组（CC）。采用荧光实时定量（Real-time quantitative PCR,q PCR）和蛋白质印迹（western blot,WB）方法分别检测胆道闭锁组（BA）和胆总管扩张组（CC）两组中肝脏组织内白介素（IL）-32的表达水平,并对两组间IL-32的表达水平进行比较分析,同时对BA组中白介素（IL）-32表达情况与肝功能相关的血清指标进行相关性分析。结果：BA组中IL-32 mRNA及蛋白表达水平明显高于CC组（P〈0.05）;BA组IL-32与γ-谷氨酰转肽酶（γ-glutamyl transpeptidase,γ-GT）、谷丙转氨酶（Alanine aminotransferase,ALT）、谷草转氨酶（Aspartate transaminase,AST）肝功能相关指标表达水平进行相关性研究表明：IL-32与γ-GT、ALT、AST之间呈正性相关的关系（P〈0.05）。结论：肝功能相关指标如γ-GT、ALT、AST的检测值随着IL-32表达水平的增高而增高,说明了胆道闭锁患儿肝组织IL-32与其肝功呈正相关的特点,对预测胆道闭锁患儿肝功能具有临床参考意义。Objective： To investigate the expression profiles and clinical values of interleukin-32 （IL-32） in liver tissues of biliary atresia （BA） group and choledochal cyst （CG）, respectively. Methords ： 35 cases of BA （observation group） and 14 cases of CC without cholangitis （control group） admitted to and surgically treated at Shenzhen Children^ Hospital from February 2011 to October 2015, were enrolled in the study. The expression level of IL-32 in liver tissues was determined and analyzed between these two group by us-ing real-time quantitative PCR （qPCR） and western blot （WB）, followed by analyzing the correlation between IL-32 and liver function index in observation group. R e su lts： The expression level of IL-32 mRNA was significantly higher in the observation group compared with the control group （P〈0.05）. In the observation group, the correlation between the expression level of IL-32 and liver function in-dex （alanine aminotransferase, i.e. ALT, aspartate transaminase, i.e. AST, and 7 -glutamyl transpeptidase, i.e. 7 -GT）, respectively, was shown to be positive （r=0.557, P =0.001, r=0.400, P =0.017, r=0.835, P =0.000, respectively）. Conclusion： The liver function index, like 7 -GT, ALT and AST were increased with the expression level of IL-32, indicating the positive correlation between IL-32 and liv-er function, which is clinical significant in predicting the infants1 liver function.

关 键 词：白介素-32 胆道闭锁 胆管扩张症 

分 类 号：R725.7[医药卫生—儿科]