长期夜班工作医护人员肠道菌群结构的变化  

Changes of intestinal flora structure in medical staff taking long-term night shift

作　　者：罗科学[1] 王钦贤[1] 陈富兴[1] 李宏春[1] 

机构地区：[1]浙江省立同德医院老年医学科,浙江杭州310032

出　　处：《中国现代医生》2017年第28期126-129,共4页China Modern Doctor

基　　金：浙江省中医药科技计划项目(2016ZA004)

摘　　要：目的调查长期夜班工作医务人员肠道菌群结构的变化,探讨肠道菌群与长期夜班工作所致慢性疾病的关系。方法采集30例有5年以上夜班工作经历的医务人员(试验组)及30例非夜班工作的健康志愿者(健康对照组)的粪便,利用荧光定量PCR方法定量分析两组人群肠道主要菌群结构的变化。结果试验组与健康对照组相比,长期夜班工作的医护人员的拟杆菌、双歧杆菌、乳酸杆菌含量均有下降,差异有高度统计学意义(P<0.01)。而肠球菌和肠杆菌则较健康对照组有所升高,其中肠球菌组间比较,差异有高度统计学意义(P<0.01),肠杆菌组间比较,差异有统计学意义(P<0.05)。结论长期夜班工作医务人员肠道内益生菌拟杆菌、双歧杆菌、乳酸杆菌减少,条件致病菌肠球菌和肠杆菌增多,存在较明显的肠道菌群结构失调。Objective To investigate the changes of intestinal flora structure in medical staff taking long-term night shift, and to explore the relationship between intestinal flora and chronic diseases caused by long-term night work.Methods Faeces of 30 cases of medical personnel with more than 5 years working experience of night work（experimental group） and 30 healthy volunteers who had no night work（healthy control group） were collected. Fluorescence quantitative PCR method was used to quantitatively analyze the changes of main intestinal flora structure in the two groups.Results Compared with the healthy control group, the contents of Bacteroides, Bifidobacterium and Lactobacillus in the medical staff taking long-term night shift were all decreased in the experimental group. There were statistically significant differences between the two groups（ P〈0.01）. The contents of Enterococcus and Enterobacteriaceae were higher than those in the healthy control group, and there was significant difference in Enterococcus between two groups（P〈0.01）. The difference in Enterobacteriaceae was significant between two groups（P〈0.05）. Conclusion The contents of intestinal probiotics such as Bacteroides, Bifidobacterium and Lactobacillus are decreased in the medical staff taking long-term night shift, while the conditioned pathogens such as Enterococcus and Enterobacteria are increased. There are more significant intestinal flora structural disorders.

关 键 词：长期夜班工作医务人员 肠道菌群 荧光定量PCR 调查 

分 类 号：R378[医药卫生—病原生物学]