间歇性张应力对小鼠破骨细胞TRPV5基因表达的影响  

Intermittent tensile stress affects the expression of TRPV5 in murine osteoclasts

作　　者：彭斐[1] 宋玲[2] 戚向敏[3] 李亚芹 刁艳菲 钊守凤[4] 王莉莉[4] 杨芳[2] 卢恕来[2] 吕文芳[2] 郭大伟[2] 

机构地区：[1]潍坊医学院,山东潍坊261000 [2]青岛市市立医院口腔科,山东青岛266071 [3]山东大学口腔医学院,山东济南250012 [4]青岛市市立医院中心实验室,山东青岛266071

出　　处：《山东大学学报（医学版）》2017年第11期22-26,共5页Journal of Shandong University:Health Sciences

基　　金：山东省医药卫生科技发展计划(2013WS0014);青岛市科技计划基础研究项目[(12-1-4-16-(8)-jch)];青岛市医疗卫生优秀人才培养项目(VDTR2016Y08)

摘　　要：目的探究在间歇性张应力作用下小鼠破骨细胞内TRPV5的基因表达情况。方法将小鼠破骨细胞分组并施加不同大小的间歇性张应力(A组1 000με、B组3 000με、C组5 000με、D组不做加力处理),通过荧光定量PCR方法检测在应力作用前后TRPV5的mRNA表达水平。结果对小鼠破骨细胞施加不同程度的间歇性张应力之后,TRPV5基因的表达水平相比对照组(D组)均升高,加力组与对照组之间差异有统计学意义(P<0.001),两两比较结果显示,B组与C组之间差异无统计学意义(P>0.05),其余任意两组之间差异均有统计学意义(P<0.05)。结论间歇性张应力可以促进破骨细胞TRPV5基因的表达,在一定范围内加大力值可提高TRPV5基因的表达。Objective To investigate the expression of transient receptor potential vanilloid receptor V（ TRPV5） during the differentiation of osteoclasts mediated by intermittent tensile stress. Methods Murine osteoclasts were divided into4 groups and mediated by different loading stress： group A（ 1 000 με）,group B（ 3 000 με）,group C（ 5 000 με）,and group D（ 0 με）. The mRNA expression of TRPV5 in the 4 groups was detected with fluorescence quantitative PCR before and after the differentiation. Results Compared with group D,the other 3 groups had higher mRNA expression of TRPV5（ P 0. 001）. Pairwise comparison results showed that there was no statistical difference between group B and C（ P 0. 05）,there was statistical difference between any other two groups（ P 0. 05）. Conclusion Intermittent tensile stress promotes the expression of TRPV5 channel gene in osteoclasts within a certain range.

关 键 词：瞬时电位感受器香草精家族受体V 间歇性张应力 破骨细胞 荧光定量PCR 

分 类 号：R782[医药卫生—口腔医学]