人脐带间充质干细胞移植治疗大鼠压力性尿失禁  

Transplanting human umbilical cord mesenchymal stem cells to treat stress urinary incontinence in rats

作　　者：蓝建发[1] 潘华[2] 罗新[3] 石海燕[3] Lan Jian-fa Pan Hua Luo Xin Shi Hai-yan(Department of Obstetrics and Gynecology Department of Clinical Laboratory, First Affiliated Hospital of Xiamen University, Xiamen 361003, China Department of Obstetrics and Gynecology, First Affiliated Hospital of Jinan University, Guangzhou 510000, China)

机构地区：[1]厦门大学附属第一医院妇产科,福建厦门361003 [2]厦门大学附属第一医院检验科,福建厦门361003 [3]暨南大学附属第一医院,广东广州510000

出　　处：《兰州大学学报（医学版）》2017年第5期1-6,共6页Journal of Lanzhou University（Medical Sciences）

基　　金：国家自然科学基金项目(81070459)

摘　　要：目的探讨人脐带间充质干细胞(HUMSCs)移植治疗大鼠压力性尿失禁(SUI)的作用机制及有效性和可行性。方法建立SUI大鼠模型,模型大鼠分为HUMSCs治疗组8只(A组,大鼠尿道周围注射HUMSCs约1×107个细胞)和实验对照组8只(B组,相同部位注射等量生理盐水),另取6只正常大鼠作为正常对照组(C组)。建模后4周,将HUMSCs移植注射于大鼠尿道周围,在移植注射前1周及移植注射后6周分别测量所有大鼠最大膀胱容量(MBC)和漏尿点压力(LPP);行免疫组织化学检查,分析大鼠尿道括约肌中结蛋白、重链肌球蛋白(MHC)和蛋白基因产物9.5(PGP9.5)的表达。结果 HUMSCs治疗组大鼠MBC、LPP测量值均较移植治疗前明显增加,差异有统计学意义(P<0.01);与实验对照组的MBC、LPP测量值相比显著增加,差异有统计学意义(P<0.01)。免疫组织化学检查显示HUMSCs治疗组大鼠尿道括约肌中结蛋白、MHC、PGP9.5含量均较实验对照组明显增多,差异有统计学意义(P<0.01)。结论 HUMSCs移植治疗SUI大鼠有效、可行,HUMSCs通过修复损伤的大鼠尿道括约肌和周围神经来提高控尿功能。Objective To explore the mechanism,effectiveness and feasibility of human umbilical cord mesenchymal stem cell（HUMSCs） transplantating therapy in rats with stress urinary incontinence（SUI）.Methods A SUI rats model were established and SUI rats were divided into two groups,8 rats in the HUMSCs treatment group and 8 in the experimental control group,with other normal rats in a normal control group.In the HUMSCs treatment group,HUMSCs were injected into the rats＇ urethra at the 4 th week,after the SUI rats model was established.The leak point pressure（LPP） and maximal bladder capacity（MBC） were quantified at the first week before transplantation and the 6 th week after it.The expression of desmin,myosin heavy chain（MHC） and protein gene product 9.5（PGP9.5） in the sphincter of rat urethra were analyzed,after the rats were sacrificed.Results The values of MBC and LPP were significantly higher than those before transplantation in HUMSCs treatment group（P〈 0.01）.In the HUMSCs treatment group,both values were significantly higher than those in the experimental control group,and the difference was statistically significant（P〈 0.01）.Immunohistochemical staining and image analysis showed that the expression of desmin,MHC and PGP9.5 in the HUMSCs group was significantly higher than that of the experimental control group（P〈 0.01）.Conclusion HUMSCs transplantation for the treatment of SUI rats is effective and feasible.HUMSCs can improve uremic function by repairing the injured urethral sphincter and peripheral nerves.

关 键 词：人脐带 间充质干细胞 压力性尿失禁 结蛋白 重链肌球蛋白 蛋白基因产物9.5 

分 类 号：R711[医药卫生—妇产科学]