尖孢镰刀菌磷脂脂肪酸的遗传多样性分析  

Genetic diversity of Fusarium oxysporum isolates revealed by phospholipid fatty acids(PLFAs) biomarkers

作　　者：肖荣凤[1] 刘波[1] 朱育菁[1] 潘志针[1] 陈梅春[1] 詹洪[1] Xiao Rongfeng Liu Bo Zhu Yujing Pan Zhizhen Chen Meichun Zhan Hong(Research Institute of Agricultural Bio-Resources, Fujian Academy of Agricultural Sciences, Fuzhou 350003, Fujian Province, Chin)

机构地区：[1]福建省农业科学院农业生物资源研究所,福州350003

出　　处：《植物保护学报》2017年第5期763-770,共8页Journal of Plant Protection

基　　金：福建省自然科学基金(2016J01128);福建省农业科技重大专项(2015NZ0003-1);福建省属公益类科研专项(2015R1018-3)

摘　　要：为明确尖孢镰刀菌Fusarium oxysporum不同专化型菌株的遗传多样性,利用MIDI微生物脂肪酸鉴定系统分析了来源于5个专化型的27株菌株的磷脂脂肪酸种类和含量,并进行了磷脂脂肪酸聚类分析。结果表明,供试尖孢镰刀菌中共检测到9种脂肪酸,在兰氏距离为25.98时分为3个类群,即主要脂肪酸、微量脂肪酸和偶现脂肪酸,其中主要脂肪酸有16:0、18:0、18:1cis9(ω9)和18:2cis9,12/18:0a,含量共占总磷脂脂肪酸的96.41%;微量脂肪酸有14:0、15:0、16:1cis9(ω7)和20:0,偶现脂肪酸仅有17:1(ω11)。不同专化型菌株的主要脂肪酸仅18:2cis9,12/18:0a存在显著差异,微量脂肪酸除15:0外其余3种均有显著差异。聚类分析结果表明,以供试的全部27株菌株聚类时,其磷脂脂肪酸与寄主专化型间无明显相关性,以寄主来源为同科(葫芦科或茄科)的菌株聚类时,其磷脂脂肪酸与寄主专化型间存在着一定的对应关系。In order to explore the genetic diversity of the formae speciales of Fusarium oxysporum, 27 isolates belonging to five formae speciales were tested with MIDI microbial phospholipid fatty acids（PLFAs） identification system. The varieties and contents of PLFAs were detected and then analyzed by using cluster method. The tested isolates had nine varieties of fatty acids, which could be divided into three groups（predominant, minor and casual fatty acids）, while the Lance distance was 25.98. The predominant fatty acids were 16：0, 18：0, 18：1 cis9（ω9） and 18：2 cis9,12/18：0 a, accounting for 96.41% of the total PLFA content, while 14：0, 15：0,16：1 cis9（ω7） and 20：0 were the minor fatty acids and 17：1（ω11）was the only casual fatty acid. Among different formae speciales, 18：2 cis9,12/18：0 a was the only predominant fatty acid that had significant difference, whereas all of the minor fatty acids differed significantly except 15：0. The cluster analysis results showed that there was no obvious correlation between fatty acid groups and host formae speciales. However, a corresponding relationship was found between phospholipid fatty acids and formae speciales among the isolates from the host crops within the same family, such as Cucurbitaceae and Solanaceae.

关 键 词：尖孢镰刀菌 专化型 磷脂脂肪酸 遗传多样性 

分 类 号：S432.44[农业科学—植物病理学]