补骨脂酚的提取纯化工艺优选及其对骨质疏松症的治疗作用分析  

Optimization of Extraction and Purification Process of Bakuchiol and Analysis of Its Effect on Osteoporosis

作　　者：杨胜杰 钟少达 杨进平 刘敏 洪达 刘明川 王昭日 YANG Sheng-jie ZHONG Shao-da YANG Jin-ping LIU Min HONG Da LIU Ming-chuan WANG Chao-jih(Institute of Sinphar Tianli Pharmaceutical Co. Ltd. （Hangzhou） , Sinphar Group, Hangzhou 311100, China)

机构地区：[1]杏辉医药集团杏辉天力(杭州)药业有限公司研究所,杭州311100

出　　处：《中国实验方剂学杂志》2017年第22期43-47,共5页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:优选补骨脂酚的提取纯化工艺,并考察其对骨质疏松症的治疗作用。方法:使用乙醇回流法提取,经D101型大孔树脂纯化,通过单因素试验优选工艺条件,冷冻干燥后得到补骨脂酚;采用斑马鱼体内试验评价补骨脂酚对骨密度和骨骼发育钙化的影响。结果:优选的工艺条件为加8倍量85%乙醇浸泡过夜,回流提取3次,每次2 h,边搅拌边往补骨脂酚粗提物中加入0.2%氢氧化钠水溶液至完全溶解,过预处理好的D101型大孔树脂柱处理,上样流速1 BV·h^(-1),静置4 h,加水6 BV洗脱,用60%乙醇2 BV洗脱,加95%乙醇4 BV洗脱,收集95%乙醇的洗脱液;制备的补骨脂酚质量分数>80%,收率>10%。斑马鱼体内活性研究表明补骨脂酚能提高斑马鱼骨质疏松症的骨密度,且能促进正常斑马鱼骨骼发育钙化。结论:此工艺具有绿色环保、操作简便等优点,所得补骨脂酚的纯度和收率均比较理想,可开发成预防或治疗骨质疏松症的药品和食品。Objective： To establish a simple and efficient method for the preparation of bakuchiol,and study on its antiosteoporotic activity. Method： The powder of Psoraleae Fructus was extracted with ethanol to get the crude extract of bakuchiol, and bakuchiol was purified with D101 macroporous resin. Moreover,antiosteoporotic activity of bakuchiol on the osteoporosis model was evaluated by zebrafish in vivo. Result： Optimum process for the purification of bakuchiol was as follows, the powder of Psoraleae Fructus was soaked in 85%ethanol overnight and the ratio of ethanol to sample was 1 ∶ 8,it was refluxed thrice for 2 h each time. The crude extract was concentrated by a vacuum rotary evaporator,and it was dissolved with 0. 2% Na OH solution,it was adsorbed by D101 macroporous resin column with a flow rate of 1 BV·h^-1,washed by 6 BV of water after 4 h and2 BV of 60% ethanol,4 BV of 95% ethanol was used to wash the column at last,and eluent was concentrated to get bakuchiol. The yield and purity of bakuchiol prepared by the method were 10% and 80% at least,respectively. In addition, the bioassay indicated that it could prevent bone loss of zebrafish induced by prednisolone,and accelerate growth of zebrafish bone. Conclusion： The method combines the advantages of generality,rapidity and simplicity with high purity. Bakuchiol can be developed into drugs and foods for preventing or treating osteoporosis.

关 键 词：补骨脂 补骨脂酚 大孔树脂 斑马鱼 骨质疏松症 依替膦酸二钠 维生素D3 

分 类 号：R283.6[医药卫生—中药学] R945[医药卫生—中医学]