甘肃红芪和黄芪总多糖含量测定对比  

Total Polysaccharides Content Determination of Hedysari Radix and Astragali Radix from Gansu Province

作　　者：王瑞海[1] 叶迎[1] 许京[1] 苗青[1] 柏冬[1] 刘丽梅[1] WANG Rui-hai YE Ying XU Jing MIAO Qing BAI Dong LIU Li-mei(Institute of Basic Theory Research of Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China)

机构地区：[1]中国中医科学院中医基础理论研究所,北京100700

出　　处：《中国实验方剂学杂志》2017年第22期77-83,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院基本科研业务费自主选题项目(YZ-1438)

摘　　要：目的:建立甘肃不同产地红芪和黄芪药材中总多糖含量测定的方法,比较不同产地二者总多糖含量,以及不同生长年限(1,2年)对2种药材总多糖含量的影响。方法:以葡萄糖为对照品,采用苯酚-硫酸显色法,在490 nm波长处测定甘肃不同产地红芪和黄芪共28批样品中总多糖含量。结果:葡萄糖在16.5~165μg与吸光度线性关系良好(r=0.999 9),红芪和黄芪平均加样回收率分别为97.43%和100.33%,RSD分别为2.1%和2.3%(n=6)。1年生红芪中总多糖平均质量分数分别为宕昌地区11.45%,武都地区5.96%,陇西地区10.97%,甘肃各地区总平均9.35%;黄芪中总多糖平均质量分数分别为宕昌地区3.69%,武都地区1.93%,陇西地区7.06%,甘肃各地区总平均4.02%;2年生红芪样品中总多糖平均质量分数分别为宕昌地区6.28%,武都地区5.82%,陇西地区6.69%,甘肃各地区总平均6.23%;黄芪中总多糖平均质量分数分别为宕昌地区7.91%,武都地区8.03%,陇西地区7.12%,甘肃各地区总平均7.73%。结论:建立的测定方法简便,准确,重复性好,可用于样品中总多糖含量测定。甘肃省5县,1市区红芪和黄芪在品种及年限对比中均有极显著性差异(P<0.01),从多糖含量分析,红芪不适合替代黄芪使用。各地1年生红芪中总多糖含量均高于黄芪,2年生红芪总多糖含量均低于黄芪。红芪以宕昌产较优,且以1年生为最好;黄芪1年生以陇西、岷县较优,2年生以武都较优。Objective： To establish a method to determine the total polysaccharides content in Hedysari Radix and Astragali Radix from different areas of Gansu province,compare their contents of total polysaccharides from different habitats,and explore the effect of different growth years（ 1,2 years） on the total polysaccharides content. Method： Total polysaccharides of Hedysari Radix and Astragali Radix from different areas of Gansu province were determined by using the phenol sulfuric acid method at 490 nm,with glucose as reference substance.Result： A good linear relationship of glucose was achieved within the range of 16. 5-165 μg（ r = 0. 999 9）,the average recoveries of Hedysari Radix and Astragali Radix were 97. 43%（ RSD 2. 1%,n = 6） and 100. 33%（ RSD2. 3%,n = 6）. For one-year-old plant,the average content of total polysaccharides in Hedysari Radix from Dangchang,Wudu,Longxi and Gansu were 11. 45%,5. 96%,10. 97% and 9. 35%,respectively. The average contents of total polysaccharides in Astragali Radix from Dangchang,Wudu,Longxi and and Gansu were 3. 69%,1. 93%,7. 06% and 4. 02%,respectively. For two-year-old plant,the average contents of total polysaccharides in Hedysari Radix from Dangchang, Wudu, Longxi and Gansu were 6. 28%,5. 82%,6. 69% and 6. 23%,respectively. The average contents of total polysaccharides in Astragali Radix from Dangchang,Wudu,Longxi and Gansu were 7. 91%,8. 03%,7. 12% and 7. 73%,respectively. Conclusion： The method is simple,accurate and reproducible,and can be used for the determination of total polysaccharides in Hedysari Radix and Astragali Radix. There was significant differences in varieties and years between the content of total polysaccharides in Hedysari Radix and Astragali Radix from 5 counties and 1 city in Gansu province（ P〈0. 01）. According to the analysis of the content of total polysaccharides,Hedysari Radix was not suitable to substitute Astragali Radix. The total polysaccharide content of one-year-old Hedysari Radix was higher than that of Astragali Ra

关 键 词：黄芪 红芪 总多糖 比色法 含量对比 葡萄糖 

分 类 号：R284.1[医药卫生—中药学]