健脾益肠散对溃疡性结肠炎大鼠结肠组织HSP70表达的影响  

Effect of Jianpi Yichang Powder on Expression Levels of HSP70 in Colon Tissues of Rats with Ulcerative Colitis

作　　者：蔺晓源[1,2] 余星[3] 雷贵玥 李志远 古娟 王敏[4] 刘杰民[5] LIN Xiao-yuan YU Xing LEI Gui-yue LI Zhi-yuan GU Juan WANG Min LIU Jie-min(Hunan University of Chinese Medicine, Changsha 410205, China The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, China Guiyang University of Chinese Medicine, Guiyang 550025, China The First Hospital of Guiyang University of Chinese Medicine, Guiyang 550001, China Guizhou Provincial People＇s Hospital, Guiyang 550002, China)

机构地区：[1]湖南中医药大学,长沙410208 [2]湖南中医药大学第一附属医院,长沙410007 [3]贵阳中医学院,贵阳550025 [4]贵阳中医学院第一附属医院,贵阳550001 [5]贵州省人民医院,贵阳550002

出　　处：《中国实验方剂学杂志》2017年第22期91-96,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81560773)

摘　　要：目的:探讨健脾益肠散对溃疡性结肠炎(UC)大鼠结肠组织热休克蛋白70(HSP70)蛋白和mRNA表达的影响。方法:将健康SPF级雄性SD大鼠60只,随机分为2组,正常组和造模组;造模组采用二硝基苯磺酸(TNBS)/乙醇法复制UC大鼠模型;待复制模型成功后将造模组随机分为5组,分别为模型组、柳氮磺吡啶组(0.3 g·kg^(-1))以及健脾益肠散高、中、低剂量组(204,136,68 g·kg^(-1)),每组10只;灌胃相应药物21 d后,观察各组大鼠的一般状态和结肠黏膜组织损伤情况,免疫组化、蛋白免疫印迹法(Western blot)和实时荧光定量PCR(Real-time PCR)分别检测大鼠结肠组织中HSP70的蛋白和mRNA表达。结果:与正常组比较,模型组大鼠结肠黏膜损伤评分显著升高(P<0.01),HSP70蛋白和mRNA表达均显著降低(P<0.01)。与模型组比较,各给药组结肠黏膜损伤评分均显著降低(P<0.01),各给药组均可增加结肠组织HSP70的蛋白和mRNA表达(P<0.05,P<0.01),其中以健脾益肠散高剂量组最为明显(P<0.05,P<0.01)。结论:健脾益肠散可能通过促进HSP70的表达而达到对UC大鼠结肠黏膜的免疫保护,从而发挥治疗作用。Objective： To explore the effect of Jianpi Yichang powder（ JP） on protein and mRNA expression levels of heat shock protein 70（ HSP70） in colon tissues of rats with ulcerative colitis（ UC）. Method：The 60 healthy SPF male SD rats were randomly divided into normal group and model group. The UC models were established by trinitro-benzene-sulfonic acid（ TNBS）/ethanol method. Then the successfully modeled rats were randomly divided into the model group,sulfasalazine group（ 0. 3 g · kg^-1）,and JP high,middle and low dose groups（ 204,136 and 68 g·kg^-1） with 10 rats in each group. After ig administration for 21 days,the general condition and the colonic mucosa injury of rats were observed. Immunohistochemistry,Western blot and Real-time PCR were used to detect the protein and mRNA expression levels of HSP70 in colon tissues of rats. Result： As compared with the normal group,the colon mucosal injury score was increased significantly（ P 0. 01）,and the protein and mRNA expression levels of HSP70 were significantly decreased（ P 0. 01） in model group. As compared with the model group,the colon mucosal injury scores in each drug group were significantly lower（ P 0. 01）,and the protein and mRNA expression levels of HSP70 were significantly increased（ P 0. 05,P 0. 01）.The effect was most obvious in JP high dose group（ P 0. 05,P 0. 01）. Conclusion： JP may promote the expression of HSP70 to protect intestinal mucosal immunity in UC rats,so as to play a therapeutic role.

关 键 词：健脾益肠散 溃疡性结肠炎 热休克蛋白70 蛋白表达 基因表达 

分 类 号：R285.5[医药卫生—中药学]